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    ZOOMICS: Comparative Metabolomics of Red Blood Cells From Old World Monkeys and Humans

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    Author
    Bertolone, Lorenzo
    Shin, Hye K.
    Stefanoni, Davide
    Baek, Jin Hyen
    Gao, Yamei
    Morrison, Evan J.
    Nemkov, Travis
    Thomas, Tiffany
    Francis, Richard O.
    Hod, Eldad A.
    Zimring, James C.
    Yoshida, Tatsuro
    Karafin, Matthew
    Schwartz, Joseph
    Hudson, Krystalyn E.
    Spitalnik, Steven L.
    Buehler, Paul W.
    D’Alessandro, Angelo
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    Date
    2020-10-23
    Journal
    Frontiers in Physiology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.3389/fphys.2020.593841
    Abstract
    As part of the ZOOMICS project, we set out to investigate common and diverging metabolic traits in the blood metabolome across various species by taking advantage of recent developments in high-throughput metabolomics. Here we provide the first comparative metabolomics analysis of fresh and stored human (n = 21, 10 males, 11 females), olive baboon (n = 20), and rhesus macaque (n = 20) red blood cells at baseline and upon 42 days of storage under blood bank conditions. The results indicated similarities and differences across species, which ultimately resulted in a differential propensity to undergo morphological alterations and lyse as a function of the duration of refrigerated storage. Focusing on purine oxidation, carboxylic acid, fatty acid, and arginine metabolism further highlighted species-specific metabolic wiring. For example, through a combination of steady state measurements and 13C615N4-arginine tracing experiments, we report an increase in arginine catabolism into ornithine in humans, suggestive of species-specific arginase 1 activity and nitric oxide synthesis—an observation that may impact the translatability of cardiovascular disease studies carried out in non-human primates (NHPs). Finally, we correlated metabolic measurements to storage-induced morphological alterations via scanning electron microscopy and hemolysis, which were significantly lower in human red cells compared to both NHPs.
    Sponsors
    National Heart, Lung, and Blood Institute
    Keyword
    blood storage
    comparative biology
    metabolomics
    red blood cell
    transfusion
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/14116
    ae974a485f413a2113503eed53cd6c53
    10.3389/fphys.2020.593841
    Scopus Count
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    UMB Open Access Articles 2020

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