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dc.contributor.authorPalmateer, Nicholas C
dc.contributor.authorTretina, Kyle
dc.contributor.authorOrvis, Joshua
dc.contributor.authorIfeonu, Olukemi O
dc.contributor.authorCrabtree, Jonathan
dc.contributor.authorDrabék, Elliott
dc.contributor.authorPelle, Roger
dc.contributor.authorAwino, Elias
dc.contributor.authorGotia, Hanzel T
dc.contributor.authorMunro, James B
dc.contributor.authorTallon, Luke
dc.contributor.authorMorrison, W Ivan
dc.contributor.authorDaubenberger, Claudia A
dc.contributor.authorNene, Vish
dc.contributor.authorKnowles, Donald P
dc.contributor.authorBishop, Richard P
dc.contributor.authorSilva, Joana C
dc.date.accessioned2020-11-10T15:31:32Z
dc.date.available2020-11-10T15:31:32Z
dc.date.issued2020-10-29
dc.identifier.urihttp://hdl.handle.net/10713/14066
dc.description.abstractTheileria parva is an economically important, intracellular, tick-transmitted parasite of cattle. A live vaccine against the parasite is effective against challenge from cattle-transmissible T. parva but not against genotypes originating from the African Cape buffalo, a major wildlife reservoir, prompting the need to characterize genome-wide variation within and between cattle-and buffalo-associated T. parva populations. Here, we describe a capture-based tar-get enrichment approach that enables, for the first time, de novo assembly of nearly com-plete T. parva genomes derived from infected host cell lines. This approach has exceptionally high specificity and sensitivity and is successful for both cattle-and buffalo-derived T. parva parasites. De novo genome assemblies generated for cattle genotypes differ from the reference by ~54K single nucleotide polymorphisms (SNPs) throughout the 8.31 Mb genome, an average of 6.5 SNPs/kb. We report the first buffalo-derived T. parva genome, which is ~20 kb larger than the genome from the reference, cattle-derived, Muguga strain, and contains 25 new potential genes. The average non-synonymous nucleotide diversity (πN) per gene, between buffalo-derived T. parva and the Muguga strain, was 1.3%. This remarkably high level of genetic divergence is supported by an average Wright’s fixa-tion index (FST), genome-wide, of 0.44, reflecting a degree of genetic differentiation between cattle-and buffalo-derived T. parva parasites more commonly seen between, rather than within, species. These findings present clear implications for vaccine development, further demonstrated by the ability to assemble nearly all known antigens in the buffalo-derived strain, which will be critical in design of next generation vaccines. The DNA capture approach used provides a clear advantage in specificity over alternative T. parva DNA enrichment methods used previously, such as those that utilize schizont purification, is less labor intensive, and enables in-depth comparative genomics in this apicomplexan parasite. © 2020 Palmateer et al.en_US
dc.description.urihttps://doi.org/10.1371/journal.pntd.0008781en_US
dc.language.isoenen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS Neglected Tropical Diseasesen_US
dc.subject.meshCattleen_US
dc.subject.meshBuffaloesen_US
dc.subject.meshTheileria parvaen_US
dc.subject.meshParasitesen_US
dc.subject.meshTicksen_US
dc.subject.meshDNAen_US
dc.subject.meshGenomicsen_US
dc.subject.meshVaccinesen_US
dc.titleCapture-based enrichment of Theileria parva DNA enables full genome assembly of first buffalo-derived strain and reveals exceptional intra-specific genetic diversity.en_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pntd.0008781
dc.identifier.pmid33119590
dc.source.volume14
dc.source.issue10
dc.source.beginpagee0008781
dc.source.endpage
dc.source.countryUnited States


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