• Login
    View Item 
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    •   UMB Digital Archive
    • School, Graduate
    • Theses and Dissertations All Schools
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    A study of ErbB receptor signal transduction pathways in a human breast cancer cell line

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Find Full text
    Author
    Yoo, Joo-Yeon
    Advisor
    Hamburger, Anne, Ph.D.
    Date
    1997
    Type
    dissertation
    
    Metadata
    Show full item record
    Abstract
    Growth factor receptors of the tyrosine kinase subfamily I, ECFR/ErbB receptor family members, EGF receptor, erbB2, erbB3, and erbB4, mediate specific biological signals via heterodimerization among family members after ligand stimulation. The overall aim of this study was to examine erbB family member interaction mediating the biological effect of Heregulin (HRG), an erbB3 ligand. C-erbB2, an orphan receptor, was involved in the concentration-dependent pleiotropic responses of a breast cancer cell line to HRG, via heterodimerization with erbB3. HRG-mediated proliferation was maintained in erbB2-nonexpressing, antisense-erbB2 stable transfectants. In contrast, c-erbB2 was required for induction of HRC-mediated differentiation at high concentrations of HRG. To further study proteins that modulate the actions of erbB3, a receptor for HRG with an impaired tyrosine kinase activity, we isolated interacting proteins using a yeast two hybrid system screening. EBP1 and EBP2 have been identified as erbB3 interacting proteins using the yeast two hybrid system. EBP1 and EBP2 interacted with the juxtamembrane domain of erbB3 in the absence of ligand activation or intrinsic tyrosine kinase activity, in vitro. EBP1 and EBP2 mRNA was expressed in normal human epithelial tissues including brain, heart, and skeletal muscle, and in human carcinomas including breast, lung, and sarcoma. Overexpression of EBP1 or EBP2 suppressed colony growth and induced differentiation in a human breast carcinoma cell line, AU565. Treatment with HRG, but not with EGF, caused dissociation of EBP1 from erbB3 in vivo. EBP1 translocated from the cytoplasm into the nucleus following HRG stimulation. These findings suggest that HRG may regulate erbB3 function in part by directly linking receptor activation to nuclear activation through the translocation of an erbB3 interacting proteins. C-erbB2 was also partly involved in 17beta-estradiol-HRG induced cell growth inhibition of ER-negative erbB2-expressing breast carcinoma cell lines.
    Description
    University of Maryland, Baltimore. Molecular and Cellular Biology. Ph.D. 1997
    Keyword
    Biology, Molecular
    Biology, Cell
    Health Sciences, Oncology
    Breast--Cancer
    Neuregulin-1
    Receptor Protein-Tyrosine Kinases
    Signal Transduction
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/1402
    Collections
    Theses and Dissertations All Schools
    Theses and Dissertations School of Medicine

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.