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dc.contributor.authorCleary, Colin M.
dc.contributor.authorMoreira, Thiago S.
dc.contributor.authorTakakura, Ana C.
dc.contributor.authorNelson, Mark T.
dc.contributor.authorLongden, Thomas A.
dc.contributor.authorMulkey, Daniel K.
dc.date.accessioned2020-10-16T18:23:02Z
dc.date.available2020-10-16T18:23:02Z
dc.date.issued2020-09-14
dc.identifier.urihttp://hdl.handle.net/10713/13884
dc.description.abstractRespiratory chemoreceptors regulate breathing in response to changes in tissue CO2/ H+. Blood flow is a fundamental determinant of tissue CO2/H+, yet little is known regarding how regulation of vascular tone in chemoreceptor regions contributes to respiratory behavior. Previously, we showed in rat that CO2/H+-vasoconstriction in the retrotrapezoid nucleus (RTN) supports chemoreception by a purinergic-dependent mechanism (Hawkins et al., 2017). Here, we show in mice that CO2/H+ dilates arterioles in other chemoreceptor regions, thus demonstrating CO2/H+ vascular reactivity in the RTN is unique. We also identify P2Y2 receptors in RTN smooth muscle cells as the substrate responsible for this response. Specifically, pharmacological blockade or genetic deletion of P2Y2 from smooth muscle cells blunted the ventilatory response to CO2, and re-expression of P2Y2 receptors only in RTN smooth muscle cells fully rescued the CO2/H+ chemoreflex. These results identify P2Y2 receptors in RTN smooth muscle cells as requisite determinants of respiratory chemoreception.en_US
dc.description.sponsorshipAmerican Heart Associationen_US
dc.description.urihttps://doi.org/10.7554/ELIFE.59499en_US
dc.language.isoenen_US
dc.publishereLife Sciences Publicationsen_US
dc.relation.ispartofeLifeen_US
dc.subjectastrocyteen_US
dc.subjectchemoreceptionen_US
dc.subjectfunctional vascular heterogeneityen_US
dc.subjectmouseen_US
dc.subjectneuroscienceen_US
dc.subjectneurovascular uniten_US
dc.subjectretrotrapezoid nucleusen_US
dc.titleVascular control of the CO2/H+ dependent drive to breatheen_US
dc.typeArticleen_US
dc.identifier.doi10.7554/eLife.59499
dc.identifier.pmid32924935
dc.source.volume9


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