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    Host and Parasite Transcriptomic Changes upon Successive Plasmodium falciparum Infections in Early Childhood

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    Author
    Bradwell, Katie R
    Coulibaly, Drissa
    Koné, Abdoulaye K
    Laurens, Matthew B
    Dembélé, Ahmadou
    Tolo, Youssouf
    Traoré, Karim
    Niangaly, Amadou
    Berry, Andrea A
    Kouriba, Bourema
    Plowe, Christopher V
    Doumbo, Ogobara K
    Lyke, Kirsten E
    Takala-Harrison, Shannon
    Thera, Mahamadou A
    Travassos, Mark A
    Serre, David
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    Date
    2020-08
    Journal
    mSystems
    Publisher
    American Society for Microbiology
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1128/mSystems.00116-20
    Abstract
    Children are highly susceptible to clinical malaria, and in regions where malaria is endemic, their immune systems must face successive encounters with Plasmodium falciparum parasites before they develop immunity, first against severe disease and later against uncomplicated malaria. Understanding cellular and molecular interactions between host and parasites during an infection could provide insights into the processes underlying this gradual acquisition of immunity, as well as to how parasites adapt to infect hosts that are successively more malaria experienced. Here, we describe methods to analyze the host and parasite gene expression profiles generated simultaneously from blood samples collected from five consecutive symptomatic P. falciparum infections in three Malian children. We show that the data generated enable statistical assessment of the proportions of (i) each white blood cell subset and (ii) the parasite developmental stages, as well as investigations of host-parasite gene coexpression. We also use the sequences generated to analyze allelic variations in transcribed regions and determine the complexity of each infection. While limited by the modest sample size, our analyses suggest that host gene expression profiles primarily clustered by individual, while the parasite gene expression profiles seemed to differentiate early from late infections. Overall, this study provides a solid framework to examine the mechanisms underlying acquisition of immunity to malaria infections using whole-blood transcriptome sequencing (RNA-seq).IMPORTANCE We show that dual RNA-seq from patient blood samples allows characterization of host/parasite interactions during malaria infections and can provide a solid framework to study the acquisition of antimalarial immunity, as well as the adaptations of P. falciparum to malaria-experienced hosts.
    Rights/Terms
    Copyright © 2020 Bradwell et al.
    Keyword
    malaria
    transcriptomics
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/13714
    ae974a485f413a2113503eed53cd6c53
    10.1128/mSystems.00116-20
    Scopus Count
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