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dc.contributor.authorClements, Jenna L.
dc.contributor.authorPohl, Franziska
dc.contributor.authorMuthupandi, Pandi
dc.contributor.authorRogers, Stephen C.
dc.contributor.authorMao, Jack
dc.contributor.authorDoctor, Allan
dc.contributor.authorBirman, Vladimir B.
dc.contributor.authorHeld, Jason M.
dc.date.accessioned2020-09-15T18:40:29Z
dc.date.available2020-09-15T18:40:29Z
dc.date.issued2020-10
dc.identifier.urihttp://hdl.handle.net/10713/13709
dc.description.abstractS-nitrosation of cysteine thiols (SNOs), commonly referred to as S-nitrosylation, is a cysteine oxoform that plays an important role in cellular signaling and impacts protein function and stability. Direct labeling of SNOs in cells with the flexibility to perform a wide range of cellular and biochemical assays remains a bottleneck as all SNO-targeted probes to date employ a single analytical modality such as biotin or a specific fluorophore. We therefore developed a clickable, alkyne-containing SNO probe ‘PBZyn’ based on the o-phosphino-benzoyl group warhead that enables multi-modal analysis via click conjugation. We demonstrate the utility of PBZyn to assay SNOs using in situ cellular imaging, protein blotting and affinity purification, as well as mass spectrometry. The flexible PBZyn probe will greatly facilitate investigation into the regulation of SNOs.en_US
dc.description.sponsorshipWashington University Research Strategic Allianceen_US
dc.description.urihttps://doi.org/10.1016/j.redox.2020.101707en_US
dc.language.isoenen_US
dc.publisherElsevier BVen_US
dc.relation.ispartofRedox Biologyen_US
dc.rights© 2020 Published by Elsevier B.V.en_US
dc.rights.urihttps://www.elsevier.com/tdm/userlicense/1.0/
dc.subjects-nitrosationen_US
dc.subjects-nitrosylationen_US
dc.subjectPhosphineen_US
dc.subjectSNOen_US
dc.subjects-nitrosothiolen_US
dc.subjectProbeen_US
dc.subjectClick chemistryen_US
dc.titleA clickable probe for versatile characterization of S-nitrosothiolsen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.redox.2020.101707
dc.source.volume37
dc.source.beginpage101707


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