Show simple item record

dc.contributor.authorChung, Matthew
dc.contributor.authorBasting, Preston J
dc.contributor.authorPatkus, Rayanna S
dc.contributor.authorGrote, Alexandra
dc.contributor.authorLuck, Ashley N
dc.contributor.authorGhedin, Elodie
dc.contributor.authorSlatko, Barton E
dc.contributor.authorMichalski, Michelle
dc.contributor.authorFoster, Jeremy M
dc.contributor.authorBergman, Casey M
dc.contributor.authorDunning Hotopp, Julie Cen_US
dc.date.accessioned2020-09-15T18:31:46Z
dc.date.available2020-09-15T18:31:46Z
dc.date.issued2020-09-02
dc.identifier.urihttp://hdl.handle.net/10713/13708
dc.description.abstractWolbachia is a genus containing obligate, intracellular endosymbionts with arthropod and nematode hosts. Numerous studies have identified differentially expressed transcripts in Wolbachia endosymbionts that potentially inform the biological interplay between these endosymbionts and their hosts, albeit with discordant results. Here, we re-analyze previously published Wolbachia RNA-Seq transcriptomics data sets using a single workflow consisting of the most up-to-date algorithms and techniques, with the aim of identifying trends or patterns in the pan-Wolbachia transcriptional response. We find that data from one of the early studies in filarial nematodes did not allow for robust conclusions about Wolbachia differential expression with these methods, suggesting the original interpretations should be reconsidered. Across datasets analyzed with this unified workflow, there is a general lack of global gene regulation with the exception of a weak transcriptional response resulting in the upregulation of ribosomal proteins in early larval stages. This weak response is observed across diverse Wolbachia strains from both nematode and insect hosts suggesting a potential pan-Wolbachia transcriptional response during host development that diverged more than 700 million years ago.en_US
dc.description.urihttps://doi.org/10.1534/g3.120.401534en_US
dc.language.isoenen_US
dc.publisherGenetics Society of Americaen_US
dc.relation.ispartofG3: Genes, Genomes, Geneticsen_US
dc.rightsCopyright © 2020 Chung et al.en_US
dc.subjectRNA-Seqen_US
dc.subjectWolbachiaen_US
dc.subjectbacteriaen_US
dc.subjectintracellularen_US
dc.subjectmeta-analysisen_US
dc.subjecttranscriptomicsen_US
dc.titleA Meta-Analysis of Transcriptomics Reveals a Stage-Specific Transcriptional Response Shared Across Different Hostsen_US
dc.typeArticleen_US
dc.identifier.doi10.1534/g3.120.401534
dc.identifier.pmid32718933
dc.source.volume10
dc.source.issue9
dc.source.beginpage3243
dc.source.endpage3260
dc.source.countryUnited States


This item appears in the following Collection(s)

Show simple item record