A Meta-Analysis of Transcriptomics Reveals a Stage-Specific Transcriptional Response Shared Across Different Hosts
Author
Chung, MatthewBasting, Preston J
Patkus, Rayanna S
Grote, Alexandra
Luck, Ashley N
Ghedin, Elodie
Slatko, Barton E
Michalski, Michelle
Foster, Jeremy M
Bergman, Casey M
Dunning Hotopp, Julie C
Date
2020-09-02Journal
G3: Genes, Genomes, GeneticsPublisher
Genetics Society of AmericaType
Article
Metadata
Show full item recordAbstract
Wolbachia is a genus containing obligate, intracellular endosymbionts with arthropod and nematode hosts. Numerous studies have identified differentially expressed transcripts in Wolbachia endosymbionts that potentially inform the biological interplay between these endosymbionts and their hosts, albeit with discordant results. Here, we re-analyze previously published Wolbachia RNA-Seq transcriptomics data sets using a single workflow consisting of the most up-to-date algorithms and techniques, with the aim of identifying trends or patterns in the pan-Wolbachia transcriptional response. We find that data from one of the early studies in filarial nematodes did not allow for robust conclusions about Wolbachia differential expression with these methods, suggesting the original interpretations should be reconsidered. Across datasets analyzed with this unified workflow, there is a general lack of global gene regulation with the exception of a weak transcriptional response resulting in the upregulation of ribosomal proteins in early larval stages. This weak response is observed across diverse Wolbachia strains from both nematode and insect hosts suggesting a potential pan-Wolbachia transcriptional response during host development that diverged more than 700 million years ago.Rights/Terms
Copyright © 2020 Chung et al.Identifier to cite or link to this item
http://hdl.handle.net/10713/13708ae974a485f413a2113503eed53cd6c53
10.1534/g3.120.401534