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dc.contributor.authorSztein, M.B.
dc.contributor.authorBafford, A.C.
dc.contributor.authorSalerno-Goncalves, R.
dc.date.accessioned2020-08-21T15:58:11Z
dc.date.available2020-08-21T15:58:11Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85089385228&doi=10.1038%2fs41598-020-70492-2&partnerID=40&md5=cc6eaf254854994f3dff175d4762316d
dc.identifier.urihttp://hdl.handle.net/10713/13569
dc.descriptionThe original version of this Article contained errors in the spelling of the authors Marcelo B. Sztein and Andrea C. Bafford which were incorrectly given as M. B. Sztein and A. C. Bafford respectively. Furthermore, in the Author Contributions section the initials of the authors R.S.-G., A.C.B. and M.B.S. were incorrectly given as R.S., A.B. and M.S. Finally, the Supplementary Information file contained errors in the spelling of the authors Marcelo B. Sztein, Andrea C. Bafford and Rosângela Salerno-Goncalves which were incorrectly given as M. B. Sztein, A. C. Bafford and R. Salerno-Goncalves respectively. These errors have now been corrected in the PDF and HTML versions of the Article, and in the accompanying Supplementary Information file.
dc.description.abstractSalmonella enterica serovar Typhi (S. Typhi) causes substantial morbidity and mortality worldwide, particularly among young children. Humans develop an array of mucosal immune responses following S. Typhi infection. Whereas the cellular mechanisms involved in S. Typhi infection have been intensively studied, very little is known about the early chromatin modifications occurring in the human gut microenvironment that influence downstream immune responses. To address this gap in knowledge, cells isolated from human terminal ileum exposed ex vivo to the wild-type S. Typhi strain were stained with a 33-metal-labeled antibody panel for mass cytometry analyses of the early chromatin modifications modulated by S. Typhi. We measured the cellular levels of 6 classes of histone modifications, and 1 histone variant in 11 major cell subsets (i.e., B, CD3 + T, CD4 + T, CD8 + T, NK, TCR-γδ, Mucosal associated invariant (MAIT), and NKT cells as well as monocytes, macrophages, and epithelial cells). We found that arginine methylation might regulate the early-differentiation of effector-memory CD4+ T-cells following exposure to S. Typhi. We also found S. Typhi-induced post-translational modifications in histone methylation and acetylation associated with epithelial cells, NKT, MAIT, TCR-γδ, Monocytes, and CD8 + T-cells that are related to both gene activation and silencing. Copyright 2020, The Author(s).en_US
dc.description.sponsorshipThis work was supported, in part, by NIAID, NIH, DHHS federal research grants R01 AI036525, U19 AI082655 (Cooperative Center for Human Immunology [CCHI]), U19-AI109776 (Center of Excellence for Translational Research [CETR], and U19 AI142725 to MS and by University of Maryland Baltimore (UMB), and Institute for Clinical & Translational Research (ICTR) to RSG.en_US
dc.description.urihttps://doi.org/10.1038/s41598-020-70492-2en_US
dc.description.urihttps://doi.org/10.1038/s41598-020-79464-y
dc.language.isoen_USen_US
dc.publisherNature Researchen_US
dc.relation.ispartofScientific Reports
dc.subject.meshSalmonella typhien_US
dc.subject.meshImmunity, Mucosalen_US
dc.subject.meshGastrointestinal Microbiomeen_US
dc.subject.meshProtein Processing, Post-Translationalen_US
dc.subject.meshCD4-Positive T-Lymphocytesen_US
dc.titleSalmonella enterica serovar Typhi exposure elicits ex vivo cell-type-specific epigenetic changes in human gut cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-020-70492-2
dc.identifier.doi10.1038/s41598-020-79464-y


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