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dc.contributor.authorLee, Rachel M
dc.contributor.authorCampanello, Leonard
dc.contributor.authorHourwitz, Matt J
dc.contributor.authorAlvarez, Phillip
dc.contributor.authorOmidvar, Ava
dc.contributor.authorFourkas, John T
dc.contributor.authorLosert, Wolfgang
dc.date.accessioned2020-08-04T17:21:48Z
dc.date.available2020-08-04T17:21:48Z
dc.date.issued2020-02-05
dc.identifier.urihttp://hdl.handle.net/10713/13462
dc.description.abstractThe dynamic rearrangement of the actin cytoskeleton is an essential component of many mechanotransduction and cellular force generation pathways. Here we use periodic surface topographies with feature sizes comparable to those of in vivo collagen fibers to measure and compare actin dynamics for two representative cell types that have markedly different migratory modes and physiological purposes: slowly migrating epithelial MCF10A cells and polarizing, fast-migrating, neutrophil-like HL60 cells. Both cell types exhibit reproducible guidance of actin waves (esotaxis) on these topographies, enabling quantitative comparisons of actin dynamics. We adapt a computer-vision algorithm, optical flow, to measure the directions of actin waves at the submicron scale. Clustering the optical flow into regions that move in similar directions enables micron-scale measurements of actin-wave speed and direction. Although the speed and morphology of actin waves differ between MCF10A and HL60 cells, the underlying actin guidance by nanotopography is similar in both cell types at the micron and submicron scales.en_US
dc.description.urihttps://www.molbiolcell.org/doi/full/10.1091/mbc.E19-11-0614en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Cell Biologyen_US
dc.relation.ispartofMolecular Biology of the Cellen_US
dc.subjectactin wavesen_US
dc.subjectcell topographyen_US
dc.subjectesotaxisen_US
dc.subject.meshActin Cytoskeletonen_US
dc.titleQuantifying topography-guided actin dynamics across scales using optical flowen_US
dc.typeArticleen_US
dc.identifier.doi10.1091/mbc.E19-11-0614
dc.identifier.pmid32023172
dc.source.volume31
dc.source.issue16
dc.source.beginpage1753
dc.source.endpage1764
dc.source.countryUnited States
dc.source.countryUnited States
dc.source.countryUnited States


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