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    Quantifying topography-guided actin dynamics across scales using optical flow

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    Author
    Lee, Rachel M
    Campanello, Leonard
    Hourwitz, Matt J
    Alvarez, Phillip
    Omidvar, Ava
    Fourkas, John T
    Losert, Wolfgang
    Date
    2020-02-05
    Journal
    Molecular Biology of the Cell
    Publisher
    American Society for Cell Biology
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://www.molbiolcell.org/doi/full/10.1091/mbc.E19-11-0614
    Abstract
    The dynamic rearrangement of the actin cytoskeleton is an essential component of many mechanotransduction and cellular force generation pathways. Here we use periodic surface topographies with feature sizes comparable to those of in vivo collagen fibers to measure and compare actin dynamics for two representative cell types that have markedly different migratory modes and physiological purposes: slowly migrating epithelial MCF10A cells and polarizing, fast-migrating, neutrophil-like HL60 cells. Both cell types exhibit reproducible guidance of actin waves (esotaxis) on these topographies, enabling quantitative comparisons of actin dynamics. We adapt a computer-vision algorithm, optical flow, to measure the directions of actin waves at the submicron scale. Clustering the optical flow into regions that move in similar directions enables micron-scale measurements of actin-wave speed and direction. Although the speed and morphology of actin waves differ between MCF10A and HL60 cells, the underlying actin guidance by nanotopography is similar in both cell types at the micron and submicron scales.
    Keyword
    actin waves
    cell topography
    esotaxis
    Actin Cytoskeleton
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/13462
    ae974a485f413a2113503eed53cd6c53
    10.1091/mbc.E19-11-0614
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    UMB Open Access Articles 2020

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