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dc.contributor.authorCarter, Claire L.
dc.contributor.authorParker, George A.
dc.contributor.authorHankey, Kim G.
dc.contributor.authorFarese, Ann M.
dc.contributor.authorMacVittie, Thomas J.
dc.contributor.authorKane, Maureen A.
dc.description.abstractRadiation-induced lung injury is a highly complex combination of pathological alterations that develop over time and severity of disease development is dose-dependent. Following exposures to lethal doses of irradiation, morbidity and mortality can occur due to a combination of edema, pneumonitis and fibrosis. Protein glycosylation has essential roles in a plethora of biological and immunological processes. Alterations in glycosylation profiles have been detected in diseases ranging from infection, inflammation and cancer. We utilized mass spectrometry imaging to spatially map N-glycans to distinct pathological alterations during the clinically latent period and at 180 days post-exposure to irradiation. Results identified alterations in a number of high mannose, hybrid and complex N-glycans that were localized to regions of mucus and alveolar-bronchiolar hyperplasia, proliferations of type 2 epithelial cells, accumulations of macrophages, edema and fibrosis. The glycosylation profiles indicate most alterations occur prior to the onset of clinical symptoms as a result of pathological manifestations. Alterations in five N-glycans were identified as a function of time post-exposure. Understanding the functional roles N-glycans play in the development of these pathologies, particularly in the accumulation of macrophages and their phenotype, may lead to new therapeutic avenues for the treatment of radiation-induced lung injury. © 2020, The Author(s).en_US
dc.description.sponsorshipU.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseasesen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.ispartofScientific Reportsen_US
dc.subject.meshGlycosylation--radiation effectsen_US
dc.subject.meshLung Injuryen_US
dc.subject.meshRadiotherapy--adverse effectsen_US
dc.titleMALDI-MSI spatially maps N-glycan alterations to histologically distinct pulmonary pathologies following irradiationen_US

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