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    Potential clinical application of an automated fluorescent microbial cell counter in the detection of urinary tract infection

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    Author
    Phillips, L.E.
    Verma, S.
    Dutta, S.K.
    Date
    2020
    Journal
    Journal of Clinical Laboratory Analysis
    Publisher
    John Wiley and Sons Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1002/jcla.23334
    Abstract
    Background: Urinary tract infections (UTI) account for millions of office visits and approximately 400 000 hospital admissions every year in the United States; as a result, the cost burden of UTI in the USA is estimated at approximately $2.8 billion. There is a great deal of interest in finding newer, faster, and more reliable methods for diagnosing UTI as compared to the standard urine culture. Methods: An automated fluorescent microbial cell counter was used to compare urine samples found to be positive for Escherichia coli UTI via cell culturing (n = 11) with UTI-negative samples (n = 10). Results: Patients with a positive urine culture had significantly higher cell count results using the microbial cell counter (1.01 × 108 cells/mL) as compared to the negative samples (2.35 × 106 cells/mL; P =.0022). Conclusions: These observations suggest that automated microbial cell counters may serve as a rapid, objective method for the detection of bacteriuria in urine samples submitted for evaluation of suspected UTI. Copyright 2020 The Authors.
    Keyword
    cellular quantification
    clinical diagnostics
    Escherichia coli
    microbiology
    urinary tract infection
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087439714&doi=10.1002%2fjcla.23334&partnerID=40&md5=46be1e170fc91e6d9655fbd3badef307; http://hdl.handle.net/10713/13310
    ae974a485f413a2113503eed53cd6c53
    10.1002/jcla.23334
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