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dc.contributor.authorAtchou, K.
dc.contributor.authorOngus, J.
dc.contributor.authorSilva, J.C.
dc.date.accessioned2020-07-07T19:59:42Z
dc.date.available2020-07-07T19:59:42Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85087028898&doi=10.3389%2ffvets.2020.00287&partnerID=40&md5=84cc17dea05d4e948d70a81a794b9eca
dc.identifier.urihttp://hdl.handle.net/10713/13232
dc.description.abstractTheileria parva is a protozoan parasite that causes East Coast fever (ECF), an economically important disease of cattle in Africa. It is transmitted mainly by the tick Rhipicephalus appendiculatus. Research efforts to develop a subunit vaccine based on parasite neutralizing antibodies and cytotoxic T-lymphocytes have met with limited success. The molecular mechanisms underlying T. parva life cycle stages in the tick vector and bovine host are poorly understood, thus limiting progress toward an effective and efficient control of ECF. Transcriptomics has been used to identify candidate vaccine antigens or markers associated with virulence and disease pathology. Therefore, characterization of gene expression throughout the parasite's life cycle should shed light on host-pathogen interactions in ECF and identify genes underlying differences in parasite stages as well as potential, novel therapeutic targets. Recently, the first gene expression profiling of T. parva was conducted for the sporoblast, sporozoite, and schizont stages. The sporozoite is infective to cattle, whereas the schizont is the major pathogenic form of the parasite. The schizont can differentiate into piroplasm, which is infective to the tick vector. The present study was designed to extend the T. parva gene expression profiling to the piroplasm stage with reference to the schizont. Pairwise comparison revealed that 3,279 of a possible 4,084 protein coding genes were differentially expressed, with 1,623 (49%) genes upregulated and 1,656 (51%) downregulated in the piroplasm relative to the schizont. In addition, over 200 genes were stage-specific. In general, there were more molecular functions, biological processes, subcellular localizations, and pathways significantly enriched in the piroplasm than in the schizont. Using known antigens as benchmarks, we identified several new potential vaccine antigens, including TP04_0076 and TP04_0640, which were highly immunogenic in naturally T. parva-infected cattle. All the candidate vaccine antigens identified have yet to be investigated for their capacity to induce protective immune response against ECF. Copyright 2020 Atchou, et. al.en_US
dc.description.sponsorshipThis research was supported by the Pan African University Institute of Basic Science, Technology and Innovation of the African Union Commission. Partial funding was also received from the BecA-ILRI Hub program and ILRI through the Africa Biosciences Challenge Fund (ABCF) program. The ABCF program is funded by the Australian Department for Foreign Affairs and Trade (DFAT) through the BecA-CSIRO partnership; the Syngenta Foundation for Sustainable Agriculture (SFSA); the Bill & Melinda Gates Foundation (BMGF); the UK Department for International Development (DFID); and the Swedish International Development Cooperation Agency (Sida).en_US
dc.description.urihttps://doi.org/10.3389/fvets.2020.00287en_US
dc.language.isoen_USen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Veterinary Science
dc.subjectpiroplasmen_US
dc.subjectschizonten_US
dc.subjectTheileria parvaen_US
dc.subjecttranscriptomeen_US
dc.subjectvaccine antigensen_US
dc.titleComparative Transcriptomics of the Bovine Apicomplexan Parasite Theileria parva Developmental Stages Reveals Massive Gene Expression Variation and Potential Vaccine Antigensen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fvets.2020.00287


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