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dc.contributor.authorBerg, Werner Mente
dc.date.accessioned2012-04-06T14:18:48Z
dc.date.available2012-04-06T14:18:48Z
dc.date.issued1999
dc.identifier.urihttp://hdl.handle.net/10713/1307
dc.descriptionUniversity of Maryland, Baltimore. Molecular and Cell Biology. Ph.D. 1999en_US
dc.description.abstractAlthough the density of the HIV envelope protein (gp160) can vary on cells and virions, there has been little investigation into how the levels affect HIV infection and cell-to-cell fusion. To address this several cell lines stably producing infectious, replication defective virions of the T-cell line adapted HIV isolate, HXB2, were made. The cell lines varied from one another in gp160 surface expression over a five-fold range, while expressing similar levels of the Gag polyprotein. As a consequence, the cell lines collectively produced virus that varied in gp160 content over a six-fold range. With the cell lines it was observed that increasing gp160 surface expression increases cell-to-cell fusion efficiency and syncytium size. With the virus preparations it was found that gp160 levels had a biphasic affect on infection efficiency. Virus preparations with gp160 levels of 10-30 molecules per virion showed that increased gp160 content correlated with increased infection efficiency. However, at gp160 levels above 50 molecules per virion there was a decrease in infection efficiency associated with increasing gp160 per virion content. Therefore, it appears that increasing gp160 surface density exerts a qualitatively different affect on infection and cell-to-cell fusion. Based upon functional analysis, several cell lines producing the gp160 molecule from the primary isolate, JR-FL were also made. These cell lines were less characterized, and yet have the potential to be useful reagents in HIV research as well.en_US
dc.language.isoen_USen_US
dc.subjectBiology, Molecularen_US
dc.subjectBiology, Cellen_US
dc.subjectBiology, Microbiologyen_US
dc.subjectinfection efficiencyen_US
dc.subject.meshHeLa Cellsen_US
dc.subject.meshHIV Envelope Protein gp160en_US
dc.subject.meshVirionen_US
dc.titleHeLa cell lines producing infectious replication-defective HIV virions with varyinggp160 densities indicate that increasing cellulargp160 levels cause an increase in cell-to-cell fusion, while increasing viralgp160 levels exert a biphasic effect on infectivityen_US
dc.title.alternativeHeLa cell lines producing infectious replication-defective HIV virions with varying gp160 densities indicate that increasing cellular gp160 levels cause an increase in cell-to-cell fusion, while increasing viral gp160 levels exert a biphasic effect on infectivityen_US
dc.typedissertationen_US
dc.contributor.advisorLewis, George K., Ph.D.
dc.identifier.ispublishedYes
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