• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2020
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2020
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    A distinct class of plant and animal viral proteins that disrupt mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Jin, H.
    Li, G.
    Zhao, R.Y.
    Date
    2020
    Journal
    Science advances
    Publisher
    American Association for the Advancement of Science
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1126/sciadv.aba3418
    Abstract
    Many animal viral proteins, e.g., Vpr of HIV-1, disrupt host mitosis by directly interrupting the mitotic entry switch Wee1-Cdc25-Cdk1. However, it is unknown whether plant viruses may use this mechanism in their pathogenesis. Here, we report that the 17K protein, encoded by barley yellow dwarf viruses and related poleroviruses, delays G2/M transition and disrupts mitosis in both host (barley) and nonhost (fission yeast, Arabidopsis thaliana, and tobacco) cells through interrupting the function of Wee1-Cdc25-CDKA/Cdc2 via direct protein-protein interactions and alteration of CDKA/Cdc2 phosphorylation. When ectopically expressed, 17K disrupts the mitosis of cultured human cells, and HIV-1 Vpr inhibits plant cell growth. Furthermore, 17K and Vpr share similar secondary structural feature and common amino acid residues required for interacting with plant CDKA. Thus, our work reveals a distinct class of mitosis regulators that are conserved between plant and animal viruses and play active roles in viral pathogenesis. Copyright 2020 The Authors.
    Keyword
    mitotic entry switch Wee1-Cdc25-Cdk1
    Viral Proteins
    Mitosis
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084787174&doi=10.1126%2fsciadv.aba3418&partnerID=40&md5=dc711798b23298b617ebc81e6b69171c; http://hdl.handle.net/10713/12888
    ae974a485f413a2113503eed53cd6c53
    10.1126/sciadv.aba3418
    Scopus Count
    Collections
    UMB Open Access Articles 2020

    entitlement

    Related articles

    • HIV-1 Vpr induces cell cycle G2 arrest in fission yeast (Schizosaccharomyces pombe) through a pathway involving regulatory and catalytic subunits of PP2A and acting on both Wee1 and Cdc25.
    • Authors: Elder RT, Yu M, Chen M, Zhu X, Yanagida M, Zhao Y
    • Issue date: 2001 Sep 1
    • Viral infections and cell cycle G2/M regulation.
    • Authors: Zhao RY, Elder RT
    • Issue date: 2005 Mar
    • spo12 is a multicopy suppressor of mcs3 that is periodically expressed in fission yeast mitosis.
    • Authors: Samuel JM, Fournier N, Simanis V, Millar JB
    • Issue date: 2000 Oct
    • The cdc25 phosphatase is essential for the G2/M phase transition in the basidiomycete yeast Ustilago maydis.
    • Authors: Sgarlata C, Pérez-Martín J
    • Issue date: 2005 Dec
    • Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited.
    • Authors: Potapova TA, Sivakumar S, Flynn JN, Li R, Gorbsky GJ
    • Issue date: 2011 Apr 15
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.