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dc.contributor.authorGao, J.
dc.contributor.authorLi, X.
dc.contributor.authorBaras, B.H.
dc.date.accessioned2020-05-26T20:41:56Z
dc.date.available2020-05-26T20:41:56Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084678905&doi=10.3892%2fijmm.2020.4598&partnerID=40&md5=9fd0c764319896cb914879431e0df1f4
dc.identifier.urihttp://hdl.handle.net/10713/12823
dc.description.abstractAmoxicillin is a common pediatric drug. However, to the best of our knowledge, the role of amoxicillin in enamel hypomineralization has not yet been fully elucidated. The aim of the present study was to assess the effects of amoxicillin on enamel mineralization, the morphology of ameloblasts, as well as the expression of kallikrein-related peptidase 4 (KLK4), and the tight junction proteins, claudin 1 (CLDN1), claudin 4 (CLDN4) and occludin (OCLN), in ameloblasts of juvenile mice. A total of 36 3-day-old Kunming mice were randomly divided into three groups. The mice were administered 0, 50 or 100 mg/kg amoxicillin by intragastric administration for 19 days. The surface morphology and calcium (Ca), phosphorous (P) and carbon contents of mandibular incisors and first molars were examined by scanning electron microscopy and energy dispersive X-ray spectroscopy. Histological changes in the ameloblasts of mandibular incisors were analyzed by hematoxylin and eosin staining. The KLK4, CLDN1, CLDN4 and OCLN expression levels of ameloblasts were observed by immunohistochemical staining. The incidence of white patches in the incisor was 100% in the 100 mg/kg amoxicillin-treated groups. A greater number of enamel defects were observed in the incisal/occlusal half of mandibular incisors/molars compared with in the cervical half in the amoxicillin-treated groups. Following phosphoric-acid treatment, the enamel rod and interrod were aligned in a disorderly manner in the amoxicillin-treated groups. Amoxicillin decreased the Ca/P ratio in the enamel of mandibular incisors and molars. More intercellular spaces among maturation ameloblasts were observed in the amoxicillin-treated groups. Amoxicillin decreased KLK4 and CLDN1, CLDN4 and OCLN expression in mature ameloblasts. The administration of amoxicillin in juvenile mice induced enamel hypomineralization, and the effects of amoxicillin on enamel hypomineralization may be mediated via multiple pathways.en_US
dc.description.sponsorshipThis study was supported by National Natural Science Foundation of China (grant no. 81602812), Fundamental Research Funds for Central Universities of China (grant no. xjj2016105), and the Shaanxi Health Family Planning Research Fund (grant no. 2016D016).en_US
dc.description.urihttps://doi.org/10.3892/ijmm.2020.4598en_US
dc.language.isoen_USen_US
dc.publisherSpandidos Publicationsen_US
dc.relation.ispartofInternational Journal of Molecular Medicine
dc.subjectAmoxicillinen_US
dc.subjectEnamel hypomineralizationen_US
dc.subjectKallikreinrelated peptidase 4en_US
dc.subjectTight junction proteinsen_US
dc.titleEffects of applying amoxicillin in juvenile mice on enamel mineralization and the expression of kallikrein-related peptidase 4 and tight junction proteins in ameloblastsen_US
dc.typeArticleen_US
dc.identifier.doi10.3892/ijmm.2020.4598


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