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dc.contributor.authorLi, L.
dc.contributor.authorShirkey, M.W.
dc.contributor.authorZhang, T.
dc.contributor.authorXiong, Y.
dc.contributor.authorPiao, W.
dc.contributor.authorSaxena, V.
dc.contributor.authorPaluskievicz, C.
dc.contributor.authorLee, Y.
dc.contributor.authorToney, N.
dc.contributor.authorCerel, B.M.
dc.contributor.authorLi, Q.
dc.contributor.authorSimon, T.
dc.contributor.authorBromberg, J.S.
dc.date.accessioned2020-05-18T19:43:57Z
dc.date.available2020-05-18T19:43:57Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85084201235&doi=10.1172%2fJCI135099&partnerID=40&md5=bb6c9d6a1afbee0418fe498e54fe6c04
dc.identifier.urihttp://hdl.handle.net/10713/12780
dc.description.abstractLymph node stromal cells (LNSCs) regulate immunity through constructing lymphocyte niches. LNSC-produced laminin α5 (Lama5) regulates CD4+ T cells but the underlying mechanisms of its functions are poorly understood. Here we show that depleting Lama5 in LNSCs resulted in decreased Lama5 protein in the LN cortical ridge (CR) and around high endothelial venules (HEVs). Lama5 depletion affected LN structure with increased HEVs, upregulated chemokines, and cell adhesion molecules, and led to greater numbers of Tregs in the T cell zone. Mouse and human T cell transendothelial migration and T cell entry into LNs were suppressed by Lama5 through the receptors α6 integrin and α-dystroglycan. During immune responses and allograft transplantation, depleting Lama5 promoted antigen-specific CD4+ T cell entry into the CR through HEVs, suppressed T cell activation, and altered T cell differentiation to suppressive regulatory phenotypes. Enhanced allograft acceptance resulted from depleting Lama5 or blockade of T cell Lama5 receptors. Lama5 and Lama4/Lama5 ratios in allografts were associated with the rejection severity. Overall, our results demonstrated that stromal Lama5 regulated immune responses through altering LN structures and T cell behaviors. This study delineated a stromal Lama5–T cell receptor axis that can be targeted for immune tolerance modulation.en_US
dc.description.sponsorshipThis study was supported by NIH grants 1R01AI114496 and R01AI062765, R6029-07, R01 HL11879, P01 AI056299, and NCI P01 CA067493.en_US
dc.description.urihttps://doi.org/10.1172/JCI135099en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.relation.ispartofJournal of Clinical Investigation
dc.subjectlymph node stromal cellsen_US
dc.subject.meshReceptors, Lamininen_US
dc.subject.meshImmunityen_US
dc.titleThe lymph node stromal laminin α5 shapes alloimmunityen_US
dc.typeArticleen_US
dc.identifier.doi10.1172/JCI135099
dc.identifier.pmid32017712


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