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dc.contributor.authorLin, Y.
dc.contributor.authorHolden, V.
dc.contributor.authorDhilipkannah, P.
dc.contributor.authorDeepak, J.
dc.contributor.authorTodd, N.W.
dc.contributor.authorJiang, F.
dc.date.accessioned2020-05-08T20:13:23Z
dc.date.available2020-05-08T20:13:23Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85083709326&doi=10.3390%2fbiomedicines8040088&partnerID=40&md5=16e5259cfc83703ef01bfcf8cb205625
dc.identifier.urihttp://hdl.handle.net/10713/12712
dc.description.abstractWe propose to systematically identify a non-coding RNA (ncRNA) profile of exfoliated bronchial epitheliums of sputum from lung cancer patients. Bronchial epithelial cells enriched from sputum of 32 lung cancer patients and 33 cancer-free smokers were analyzed by next-generation sequencing to comprehensively characterize the ncRNA profiles. In addition, 108 miRNAs, 88 small nucleolar RNAs, 13 piwi-interacting RNAs, 6 transfer RNAs, 4 ribosomal RNAs, 19 small nuclear RNAs, and 25 long-noncoding (lnc) RNAs displayed a significantly different level in bronchial epitheliums of sputum of lung cancer patients versus cancer-free smokers (all < 0.001). PCR analysis confirmed their different expression levels in the sputum specimens. A high expression of SNHG9, an lncRNA, was validated in 78 lung tumor tissues, and the expression was inversely associated with overall survival of lung cancer patients (p = 0.002). Knockdown of SNHG9 in cancer cells reduced the cell growth, proliferation, and invasion in vitro and tumorigenesis in vivo. The multiple differentially expressed ncRNAs in bronchial epitheliums may contribute to the development and progression of lung cancer and provide potential biomarkers and therapeutic targets for the disease. Copyright 2020 by the authors.en_US
dc.description.sponsorshipThis work was supported in part by VA Merit Award I01 CX000512, NCI 1R21CA240556, the Geaton and JoAnn DeCesaris Family Foundation, University of Maryland Marlene & Stewart Greenebaum Comprehensive Cancer Center Pilot Grant Program, and DoD-Idea Development Award (F.J.).en_US
dc.description.urihttps://doi.org/10.3390/biomedicines8040088en_US
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofBiomedicines
dc.subjectFunctionen_US
dc.subjectBiomarkersen_US
dc.subjectLung canceren_US
dc.subjectNon-coding RNAsen_US
dc.subjectSputumen_US
dc.titleA non-coding RNA landscape of bronchial epitheliums of lung cancer patientsen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/biomedicines8040088


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