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dc.contributor.authorAugustus, E.
dc.contributor.authorVan Casteren, K.
dc.contributor.authorRolfo, C.
dc.date.accessioned2020-04-21T19:42:23Z
dc.date.available2020-04-21T19:42:23Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85083014302&doi=10.1371%2fjournal.pone.0231058&partnerID=40&md5=60f8a8990589b13f51f5c69c7ea9ec6d
dc.identifier.urihttp://hdl.handle.net/10713/12614
dc.description.abstractAlthough liquid biopsies offer many advantages over tissue biopsies, they are not yet standard practice. An important reason for the lack of implementation is the unavailability of well standardized techniques and guidelines, especially for pre-analytical conditions which are an important factor causing the current sensitivity issues. To overcome these limitations, we investigated the effect of several pre-analytical conditions on the concentration of cell-free DNA (cfDNA) and cellular genomic DNA (gDNA) contamination. Urine samples from healthy volunteers (HVs) and cancer patients were collected and processed according to specific pre-analytical conditions. Our results show that in samples with a relatively small volume more than 50% of the cfDNA can be found in the first 50 mL of the urine sample. The total DNA concentration increased again when samples were collected more than 3.5 hours apart. Adding preservative to urine samples is recommended to obtain high concentrations of cfDNA. To remove the cellular content, high speed centrifugation protocols as 4,000g 10min or 3,000g 15min are ideal for urine collected in cfDNA Urine Preserve (Streck). Although this study was a pilot study and needs to be confirmed in a larger study population, clear trends in the effect of several pre-analytical conditions were observed. Copyright 2020 Augustus et al.en_US
dc.description.sponsorshipResearch project funded by Kom Op Tegen Kanker (Stand up to Cancer), the Flemish cancer society. https://www.komoptegenkanker.be.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0231058en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONE
dc.subjectcellular genomic DNAen_US
dc.subject.meshCell-Free Nucleic Acidsen_US
dc.subject.meshUrineen_US
dc.subject.meshLiquid Biopsyen_US
dc.titleThe art of obtaining a high yield of cell-free DNA from urineen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0231058
dc.identifier.pmid32251424


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