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dc.contributor.authorTurri, V.
dc.contributor.authorLatinovic, O.S.
dc.contributor.authorToyang, N.
dc.date.accessioned2020-04-14T14:28:51Z
dc.date.available2020-04-14T14:28:51Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85082880361&doi=10.1155%2f2020%2f7465242&partnerID=40&md5=a3f8fc3614713245ba51cf1105b50512
dc.identifier.urihttp://hdl.handle.net/10713/12550
dc.description.abstractRecent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells. Copyright 2020 Valentina Turri et al.en_US
dc.description.sponsorshipPaul G. Allen Family Foundation: 12147en_US
dc.description.urihttps://doi.org/10.1155/2020/7465242en_US
dc.language.isoen_USen_US
dc.publisherHindawi Limiteden_US
dc.relation.ispartofBioMed Research International
dc.subjecttransactivator/viroplasmin proteinen_US
dc.subjectribonuclease H1en_US
dc.subjecthuman glioma cellsen_US
dc.subject.meshCaulimovirusen_US
dc.subject.meshPlant Virusesen_US
dc.subject.meshHumansen_US
dc.subject.meshCytotoxinsen_US
dc.titleCauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cellsen_US
dc.typeArticleen_US
dc.identifier.doi10.1155/2020/7465242


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