Show simple item record

dc.contributor.authorAnantha, Ravi Prakash
dc.date.accessioned2012-04-04T13:57:15Z
dc.date.available2012-04-04T13:57:15Z
dc.date.issued2000
dc.identifier.urihttp://hdl.handle.net/10713/1243
dc.descriptionUniversity of Maryland, Baltimore. Molecular and Cellular Biology. Ph.D. 2000en_US
dc.description.abstractEnteropathogenic Escherichia coli (EPEC) express a type IV fimbria known as the bundle-forming pilus (BFP). This pilus is required for bacterial autoaggregation and adherence to epithelial cells in a distinctive pattern called localized adherence (LA). EPEC strains that express BFP have a large plasmid. A cluster of fourteen genes on this plasmid is sufficient to reconstitute pilus biogenesis and localized adherence in a laboratory strain of E. coli. Type IV pilus biogenesis is a poorly understood process, and the identification of all the genes required for BFP expression in EPEC makes this an attractive system to study. The first gene in the cluster, bfpA, encodes bundlin, the major structural subunit of BFP. Bundlin is expressed as a pre-protein with a characteristic type IV pilin leader sequence. We have undertaken a systematic mutagenesis of the individual genes of the bfp cluster to determine which genes are required for BFP biogenesis, LA, and autoaggregation. Here we report the construction and analysis of nonpolar mutations in seven bfp genes, bfpG, bfpB, bfpC, bfpD, bfpF, bfpP, and bfpH, as well as the further analysis of a previously described bfpA mutant that is unable to express bundlin. The mutation in bfpP, the gene encoding the pre-pilin peptidase, does not affect pre-bundlin expression, but blocks pre-bundlin processing, BFP biogenesis, LA, and autoaggregation. The mutations in bfpG, bfpB, bfpC, and bfpD do not affect pre-bundlin expression or processing, but block BFP biogenesis, LA, and autoaggregation. The mutation in bfpF does not block any of these events, and in fact bfpF mutant strains adhere to epithelial cells in greater numbers than do wild-type EPEC. The mutation in bfpH has no discernable effect on BFP expression or function. We also show by sucrose density floatation gradient analysis that the association of prebundlin or bundlin with sucrose density floatation gradient fractions containing both inner and outer membrane proteins does not require any other Bfp proteins. Finally, we show that BfpC is a bitopic inner membrane protein. These results show that BfpP is the only prepilin peptidase in EPEC capable of processing prebundlin, BfpG, BfpB, BfpC, and BfpD are all required for BFP expression, BfpF is not required for BFP expression but does play a role in BFP function, and the bfpH gene is not required for any of the phenotypes examined here.en_US
dc.language.isoen_USen_US
dc.subjectBiology, Molecularen_US
dc.subjectBiology, Microbiologyen_US
dc.subjectbundle-forming pilus biogenesisen_US
dc.subjecttype IV pilusen_US
dc.subject.meshEnteropathogenic Escherichia coli--geneticsen_US
dc.subject.meshFimbriae, Bacterial--geneticsen_US
dc.titleCharacterization of the genes required for the expression of a type IV pilus in enteropathogenic Escherichia colien_US
dc.typedissertationen_US
dc.contributor.advisorDonnenberg, Michael S.
dc.identifier.ispublishedYes
 Find Full text

This item appears in the following Collection(s)

Show simple item record