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dc.contributor.authorColeman, C.M.
dc.contributor.authorHalasz, G.
dc.contributor.authorZhong, J.
dc.contributor.authorBeck, S.E.
dc.contributor.authorMatthews, K.L.
dc.contributor.authorVenkataraman, T.
dc.contributor.authorRajagopalan, S.
dc.contributor.authorFrieman, M.B.
dc.date.accessioned2020-03-27T15:13:13Z
dc.date.available2020-03-27T15:13:13Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85008178769&doi=10.1128%2fJVI.01825-16&partnerID=40&md5=cd94a5981af40f6e77202b05de486674
dc.identifier.urihttp://hdl.handle.net/10713/12396
dc.description.abstractMiddle East respiratory syndrome coronavirus (MERS-CoV) is an important emerging pathogen that was first described in 2012. While the cell surface receptor for MERS-CoV has been identified as dipeptidyl peptidase 4 (DPP4), the mouse DPP4 homologue does not allow virus entry into cells. Therefore, development of mouse models of MERS-CoV has been hampered by the fact that MERS-CoV does not replicate in commonly available mouse strains. We have previously described a mouse model in which mDPP4 was replaced with hDPP4 such that hDPP4 is expressed under the endogenous mDPP4 promoter. In this study, we used this mouse model to analyze the host response to MERS-CoV infection using immunological assays and transcriptome analysis. Depletion of CD4+ T cells, CD8+ T cells, or macrophages has no effect on MERS-CoV replication in the lungs of infected mice. However, we found that depletion of CD8+ T cells protects and depletion of macrophages exacerbates MERS-CoV-induced pathology and clinical symptoms of disease. Overall, we demonstrate an important role for the inflammatory response in regulating MERS-CoV pathogenesis in vivo.en_US
dc.description.sponsorshipThis work was supported in part by NIH grant R01 AI095569 to M.B.F., NIH grant T32 AI095190 to T.V., NIH grant F32 AI118303 to J.M.S., NIH grant R01 HL127422 to S.R., and Regeneron Pharmaceuticals.en_US
dc.description.urihttps://doi.org/10.1128/JVI.01825-16en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofJournal of Virology
dc.subjectCoronavirusen_US
dc.subjectDPP4en_US
dc.subjectImmune responseen_US
dc.subjectMERSen_US
dc.subjectMERS-CoVen_US
dc.subjectMouse modelen_US
dc.subjectPathogenesisen_US
dc.subjectViral pathogenesisen_US
dc.titleCD8+ T cells and macrophages regulate pathogenesis in a mouse model of Middle East respiratory syndromeen_US
dc.typeArticleen_US
dc.identifier.doi10.1128/JVI.01825-16
dc.identifier.pmid27795435
dc.identifier.ispublishedNo


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