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dc.contributor.authorTretina, K.
dc.contributor.authorHaidar, M.
dc.contributor.authorSilva, J.C.
dc.date.accessioned2020-03-23T15:54:03Z
dc.date.available2020-03-23T15:54:03Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85081197743&doi=10.1038%2fs41598-020-60939-x&partnerID=40&md5=7fc639a578f047797f3551e8a775e8e0
dc.identifier.urihttp://hdl.handle.net/10713/12304
dc.description.abstractIntracellular pathogens have evolved intricate mechanisms to subvert host cell signaling pathways and ensure their own propagation. A lineage of the protozoan parasite genus Theileria infects bovine leukocytes and induces their uncontrolled proliferation causing a leukemia-like disease. Given the importance of E2F transcription factors in mammalian cell cycle regulation, we investigated the role of E2F signaling in Theileria-induced host cell proliferation. Using comparative genomics and surface plasmon resonance, we identified parasite-derived peptides that have the sequence-specific ability to increase E2F signaling by binding E2F negative regulator Retinoblastoma-1 (RB). Using these peptides as a tool to probe host E2F signaling, we show that the disruption of RB complexes ex vivo leads to activation of E2F-driven transcription and increased leukocyte proliferation in an infection-dependent manner. This result is consistent with existing models and, together, they support a critical role of E2F signaling for Theileria-induced host cell proliferation, and its potential direct manipulation by one or more parasite proteins. Copyright 2020, The Author(s).en_US
dc.description.sponsorshipThis work was supported in part by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS) (58-5348-4-013), by the Norman Borlaug Commemorative Research Initiative, an initiative between the Feed the Future program of the U.S. Agency for International Development (USAID) and USDA-ARS (58-5348-2-117 F), by the Department for International Development of the United Kingdom, by the Bill and Melinda Gates Foundation (OPP1078791), and by the National Institute of Allergy and Infectious Diseases (T32 AI007540-14, on Immunity and Infection). This work was also supported by the grant Labex ParaFrap (ANR?11?LABX?0024) and core funding from INSERM and the CNRS awarded to GL and a CRG4 grant (URF/1/2610?01?01) from the Office for Sponsored Research (OSR) in King Abdullah University of Science and Technology (KAUST) award to AP and GL and the faculty baseline fund (BAS/1/1020?01?01) awarded to AP.en_US
dc.description.urihttps://doi.org/10.1038/s41598-020-60939-xen_US
dc.language.isoen_USen_US
dc.publisherNature Researchen_US
dc.relation.ispartofScientific Reports
dc.subject.meshE2F Transcription Factorsen_US
dc.subject.meshTheileria--pathogenicityen_US
dc.titleTheileria parasites subvert E2F signaling to stimulate leukocyte proliferationen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-020-60939-x
dc.identifier.pmid32132598


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