The antibacterial effects of quaternary ammonium salts in the simulated presence of inhibitors in root canals: A preliminary in-vitro study
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AbstractTo investigate the antibacterial effects of two newly developed quaternary ammonium salts (QAMs)-dimethylaminododecyl methacrylate (DMADDM) and dimethylaminohexadecyl methacrylate (DMAHDM), in the presence of various root canal inhibitors. Streptococcus gordonii, Enterococcus faecalis, Lactobacillus acidophilus and Actinomyces naeslundii were used. Dentine, dentine matrix and dead bacteria were selected as inhibitors. The antimicrobial effcacy of monomers of DMADDMandDMAHDMwas tested against suspensions formed by mixtures of four bacterial species in or without the presence of inhibitors. The inhibition results were compared with chlorhexidine (CHX) and sodium hypochlorite (NaOCl). One-way analyses of variance (ANOVA) followed by Tukey's multiple comparison test was performed to determine significant differences. The antibacterial effects of DMADDM and DMAHDM were variably inhibited dentine, dentine matrix and dead bacteria. CHX and NaOCl showed substantivity and they inhibited bacteria present in suspension. The concentration of compound decreased in the dentine block due to constant release. Bacterial colonies on the dentine surface and dentine tubules were significantly inhibited by DMADDM and DMAHDM. The antibacterial effects of DMADDM and DMAHDM could be inactivated by dentine, dentine matrix and dead bacteria, while DMADDM and DMAHDM could inhibit bacteria colonization on the dentine surface and kill bacteria present in dentinal tubules. The antibacterial effects of DMADDM and DMAHDM as free monomers in the presence of inhibitors was evaluated for the first time. They could help inhibit the residual bacteria on the dentine surface and in dentinal tubules that may cause persisting infection. Therefore the novel QAMs showed great potentials as root canal medication. Copyright 2020 by the authors.
SponsorsThis study was supported by National Key Research Program of China 2017YFC0840100 and 2017YFC0840107 (L. C); National Natural Science Foundation of China grant 81900993 (S. W) and 81870759 (L. C); International Science and Technology Cooperation Program of Sichuan Province 2017HH0008 (L. C); Innovative Research Team Program of Sichuan Province (L. C); and Key Science and Technology Program of Henan Province 22170123 (X. Y).
Quaternary ammonium salt
Root canal inhibitors
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85080880717&doi=10.3390%2fcoatings10020181&partnerID=40&md5=f490da1354f1333e82c627a05995c96a; http://hdl.handle.net/10713/12238