Show simple item record

dc.contributor.authorMukhopadhyay, S.
dc.contributor.authorGabre, J.
dc.contributor.authorChabasse, C.
dc.contributor.authorBromberg, J.S.
dc.contributor.authorAntalis, T.M.
dc.contributor.authorSarkar, R.
dc.date.accessioned2020-03-16T15:58:25Z
dc.date.available2020-03-16T15:58:25Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85080888181&doi=10.3390%2fijms21051650&partnerID=40&md5=d38805b3ee5a2c15cc7738238cebf026
dc.identifier.urihttp://hdl.handle.net/10713/12235
dc.description.abstractResolution of deep venous thrombosis involves coordinated inflammatory processes. T cells regulate inflammation in vivo and modulate vascular remodeling in other settings, but their role in venous thrombus resolution remains undefined. To determine the role of T cells in venous thrombus resolution in vivo, stasis induced thrombi were created by vena cava ligation in outbred CD?1 mice. CD4 and CD8 positive T cells, as determined by flow cytometry, were present in thrombi both during thrombus formation and resolution. Depletion of the CD4 and CD8 positive T cells by antibody treatment selectively impaired thrombus resolution compared to animals treated with isotype control antibodies, without an effect on venous thrombus formation. Quantitation of intra-thrombus macrophage numbers, fibrinolytic marker expression, and gelatinolytic activity by zymography revealed that T cell depletion decreased the number of macrophages, reduced the expression of fibrinolytic marker urokinase plasminogen activator (uPA), and decreased the activity of matrix metalloprotinease?9 (MMP?9). These data implicate CD4 and CD8 positive T cells in functionally contributing to venous thrombus resolution, thus representing a potential therapeutic target, but also underscoring potential risks involved in T cell depletion used clinically for solid organ and hematopoietic transplantation procedures. Copyright 2020 by the authors.en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health, National Heart, Lung, and Blood Institute grant R01 HL083917 (to R.S.), and also in part by VA Maryland Health Care System Merit award I01 BX001921 (to T.M.A.).en_US
dc.description.urihttps://doi.org/10.3390/ijms21051650en_US
dc.language.isoen_USen_US
dc.publisherMDPI AGen_US
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.subjectCD4en_US
dc.subjectCD8en_US
dc.subjectMacrophageen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectPeripheral vascular diseaseen_US
dc.subjectT cellsen_US
dc.subjectThrombus resolutionen_US
dc.titleDepletion of CD4 and CD8 positive T Cells impairs venous thrombus resolution in miceen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ijms21051650
dc.identifier.pmid32121269


This item appears in the following Collection(s)

Show simple item record