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dc.contributor.authorChibucos, M.
dc.contributor.authorMunro, J.B.
dc.contributor.authorDaugherty, S.
dc.contributor.authorSilva, J.C.en_US
dc.date.accessioned2020-02-12T15:44:56Z
dc.date.available2020-02-12T15:44:56Z
dc.date.issued2020
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077976677&doi=10.1038%2fs41598-019-56950-6&partnerID=40&md5=641620908b155a566c3611f84522e23b
dc.identifier.urihttp://hdl.handle.net/10713/11995
dc.description.abstractOlfactory receptors (ORs), encoded by the largest vertebrate multigene family, enable the detection of thousands of unique odorants in the environment and consequently play a critical role in species survival. Here, we advance our knowledge of OR gene evolution in procellariiform seabirds, an avian group which relies on the sense of olfaction for critical ecological functions. We built a cosmid library of Cory’s Shearwater (Calonectris borealis) genomic DNA, a model species for the study of olfaction-based navigation, and sequence OR gene-positive cosmid clones with a combination of sequencing technologies. We identified 220 OR open reading frames, 20 of which are full length, intact OR genes, and found a large ratio of partial and pseudogenes to intact OR genes (2:1), suggestive of a dynamic mode of evolution. Phylogenetic analyses revealed that while a few genes cluster with those of other sauropsid species in a γ (gamma) clade that predates the divergence of different avian lineages, most genes belong to an avian-specific γ-c clade, within which sequences cluster by species, suggesting frequent duplication and/or gene conversion events. We identified evidence of positive selection on full length γ-c clade genes. These patterns are consistent with a key role of adaptation in the functional diversification of olfactory receptor genes in a bird lineage that relies extensively on olfaction. Copyright 2020, The Author(s).en_US
dc.description.sponsorshipThis work was financed by Fundação para a Ciência e a Tecnologia (FCT), Portugal, through research project PTDC/ BIA-BDE/100118/2008. MCS was supported by a post-doctoral research fellowship SFRH/BPD/85700/2012 from FCT, Portugal. JCS and JBM were supported in part by NSF’s Dimensions of Biodiversity program grant OCE-1046371.en_US
dc.description.urihttps://doi.org/10.1038/s41598-019-56950-6en_US
dc.language.isoen_USen_US
dc.publisherNature Researchen_US
dc.relation.ispartofScientific Reports
dc.subjectadaptive evolutionen_US
dc.subjectCalonectris borealisen_US
dc.subjectCorey's Shearwateren_US
dc.subject.meshAdaptation, Physiologicalen_US
dc.subject.meshEvolution, Molecularen_US
dc.subject.meshGenomeen_US
dc.subject.meshReceptors, Oderanten_US
dc.titleSignature of adaptive evolution in olfactory receptor genes in Cory’s Shearwater supports molecular basis for smell in procellariiform seabirdsen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-019-56950-6
dc.identifier.pmid31953474


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