SUR1-TRPM4 channels, not K(ATP), mediate brain swelling following cerebral ischemia
MetadataShow full item record
AbstractBACKGROUND: Preclinical and emerging clinical data show that glibenclamide reduces space occupying edema and brain swelling following cerebral ischemia. Glibenclamide is a potent inhibitor of numerous sulfonylurea receptor (SUR)-regulated channels, including KATP (SUR1-KIR6.2, SUR2A-KIR6.2, SUR2B-KIR6.2, SUR2B-KIR6.1) and SUR1-TRPM4. Here, we used molecularly specific oligodeoxynucleotides (ODNs) to investigate the role of various SUR-regulated ion channel subunits in post-ischemic brain swelling. METHODS: Focal cerebral ischemia was induced in adult male rats by permanent middle cerebral artery occlusion (pMCAo). We used this model to study the effects of antisense-ODNs (AS-ODNs) directed against Abcc8/SUR1, Trpm4/TRPM4, Kcnj8/KIR6.1 and Kcnj11/KIR6.2 on hemispheric swelling, with sense or scrambled ODNs used as controls. We used antibody-based Förster resonance energy transfer (immuno-FRET) and co-immunoprecipitation to study the co-assembly of SUR1-TRPM4 heteromers. RESULTS: In the combined control groups administered sense or scrambled ODNs, pMCAo resulted in uniformly large infarct volumes (mean ± SD: 57.4 ± 8.8 %; n = 34) at 24 h after onset of ischemia, with no effect of AS-ODNs on infarct size. In controls, hemispheric swelling was 23.9 ± 4.1 % (n = 34), and swelling was linearly related to infarct volume (P < 0.02). In the groups administered anti-Abcc8/SUR1 or anti-Trpm4/TRPM4 AS-ODN, hemispheric swelling was significantly less, 11.6 ± 3.9 % and 12.8 ± 5.8 % respectively (P < 0.0001), and the relationship between infarct volume and swelling was reduced and not significant. AS-ODNs directed against Kcnj8/KIR6.1 and Kcnj11/KIR6.2 had no significant effect on hemispheric swelling (23.3 ± 5.4 % and 22.9 ± 5.8 % respectively). Post-ischemic tissues showed co-assembly of SUR1-TRPM4 heteromers. CONCLUSIONS: Post-ischemic hemispheric swelling can be decoupled from infarct volume. SUR1-TRPM4 channels, not KATP, mediate post-ischemic brain swelling. Copyright 2019 The Author(s).
SponsorsDepartment of Veterans Affairs (I01BX002889), the Department of Defense (SCI170199), the National Heart, Lung and Blood Institute (R01HL082517) and the National Institute of Neurological Disorders and Stroke (NINDS) (R01NS060801; R01NS102589; R01NS105633);NINDS (R01NS061934; R01NS107262)
Sulfonylurea receptor 1 (SUR1)
Transient receptor potential melastatin 4 (TRPM4)
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85078390913&doi=10.1016%2fj.neulet.2019.134729&partnerID=40&md5=8ed65b3920f0073f4b36ade2bf405a42; http://hdl.handle.net/10713/11654
- Sulfonylurea Receptor 1, Transient Receptor Potential Cation Channel Subfamily M Member 4, and KIR6.2:Role in Hemorrhagic Progression of Contusion.
- Authors: Gerzanich V, Stokum JA, Ivanova S, Woo SK, Tsymbalyuk O, Sharma A, Akkentli F, Imran Z, Aarabi B, Sahuquillo J, Simard JM
- Issue date: 2019 Apr 1
- Profile of intravenous glyburide for the prevention of cerebral edema following large hemispheric infarction: evidence to date.
- Authors: King ZA, Sheth KN, Kimberly WT, Simard JM
- Issue date: 2018
- On potential interactions between non-selective cation channel TRPM4 and sulfonylurea receptor SUR1.
- Authors: Sala-Rabanal M, Wang S, Nichols CG
- Issue date: 2012 Mar 16
- Sensitivity of KATP channels to cellular metabolic disorders and the underlying structural basis.
- Authors: Li CG, Cui WY, Wang H
- Issue date: 2016 Jan
- The Kir6.2-F333I mutation differentially modulates KATP channels composed of SUR1 or SUR2 subunits.
- Authors: Tammaro P, Ashcroft F
- Issue date: 2007 Jun 15