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dc.contributor.authorMoyer, A.L.
dc.contributor.authorLovering, R.
dc.contributor.authorRovira Gonzalez, Y.I.en_US
dc.date.accessioned2020-01-16T14:39:56Z
dc.date.available2020-01-16T14:39:56Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85077396751&doi=10.1172%2fjci.insight.122247&partnerID=40&md5=5b5f9d512fa0af7c4c1ea0e340785ea5
dc.identifier.urihttp://hdl.handle.net/10713/11633
dc.description.abstractMyostatin is a negative regulator of muscle growth and metabolism and its inhibition in mice improves insulin sensitivity, increases glucose uptake into skeletal muscle, and decreases total body fat. A recently described mammalian protein called MSS51 is significantly downregulated with myostatin inhibition. In vitro disruption of Mss51 results in increased levels of ATP, ?-oxidation, glycolysis, and oxidative phosphorylation. To determine the in vivo biological function of Mss51 in mice, we disrupted the Mss51 gene by CRISPR/Cas9 and found that Mss51-KO mice have normal muscle weights and fiber-type distribution but reduced fat pads. Myofibers isolated from Mss51-KO mice showed an increased oxygen consumption rate compared with WT controls, indicating an accelerated rate of skeletal muscle metabolism. The expression of genes related to oxidative phosphorylation and fatty acid ?-oxidation were enhanced in skeletal muscle of Mss51-KO mice compared with that of WT mice. We found that mice lacking Mss51 and challenged with a high-fat diet were resistant to diet-induced weight gain, had increased whole-body glucose turnover and glycolysis rate, and increased systemic insulin sensitivity and fatty acid ?-oxidation. These findings demonstrate that MSS51 modulates skeletal muscle mitochondrial respiration and regulates whole-body glucose and fatty acid metabolism, making it a potential target for obesity and diabetes.en_US
dc.description.sponsorshipNational Institutes of Health, NIH: 5U2C-DK093000en_US
dc.description.urihttps://doi.org/10.1172/jci.insight.122247en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.relation.ispartofJCI Insight
dc.subjectMSS51en_US
dc.subject.meshGlucoseen_US
dc.subject.meshHomeostasisen_US
dc.subject.meshMiceen_US
dc.subject.meshMyostatinen_US
dc.titleMss51 deletion enhances muscle metabolism and glucose homeostasis in miceen_US
dc.typeArticleen_US
dc.identifier.doi10.1172/jci.insight.122247
dc.identifier.pmid31527314


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