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dc.contributor.authorChen, H.
dc.contributor.authorTang, Y.
dc.contributor.authorWeir, M.D.
dc.contributor.authorMasri, R.
dc.contributor.authorOates, T.W.
dc.contributor.authorZhang, K.
dc.contributor.authorXu, H.H.K.
dc.date.accessioned2019-12-30T15:32:56Z
dc.date.available2019-12-30T15:32:56Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85076815704&doi=10.1039%2fc9ra09220j&partnerID=40&md5=b213f00ba0beed44e81dafb6e0de2444
dc.identifier.urihttp://hdl.handle.net/10713/11559
dc.description.abstractSecondary caries at the restoration-tooth margins is a main reason for dental restoration failures. Gene-modification for Streptococcus mutans (S. mutans) and composites containing dimethylaminohexadecyl methacrylate (DMAHDMA) and nanoparticles of amorphous calcium phosphate (NACP) all have the potential to suppress bacterial acids and promote remineralization. However, there has been no report of their effects on marginal caries-inhibition and enamel hardness. The objective of this study was to investigate the effects of gene-modification and DMAHDM-NACP composite restoration on enamel demineralization and hardness at the margins under biofilm acids for the first time. Parent S. mutans and rnc gene-deleted S. mutans were tested side by side. The bioactive composite contained 3% DMAHDM and 30% NACP. Mechanical properties and calcium (Ca) and phosphate (P) ion releases were measured. Colony-forming units (CFU), MTT, lactic acid and polysaccharide of biofilms were evaluated. Demineralization of bovine enamel with composite restorations was induced via biofilms, then enamel hardness was measured. The dual strategy of combining rnc-deletion with DMAHDM+30NACP: (1) achieved the strongest biofilm-inhibition, with the greatest reduction in biofilm CFU by 6 logs; (2) decreased biofilm lactic acid and polysaccharide production by more than 80%; (3) achieved enamel hardness that was 140% higher than that of a commercial fluoride-releasing composite under 30 days of biofilm acids. Therefore, the novel dual approach of rnc gene-deletion and DMAHDM+NACP nanocomposite is promising to inhibit secondary caries at the margins and increase the longevity of tooth restorations.en_US
dc.description.urihttps://doi.org/10.1039/c9ra09220jen_US
dc.language.isoen_USen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.ispartofRSC Advances
dc.subjectantibacterial calcium phosphate nanocompositeen_US
dc.subject.meshDental Caries--prevention & controlen_US
dc.subject.meshDental Restoration Failureen_US
dc.subject.meshStreptococcus mutans--geneticsen_US
dc.titleEffects of S. mutans gene-modification and antibacterial calcium phosphate nanocomposite on secondary caries and marginal enamel hardnessen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/c9ra09220j


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