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    Effect of Physiologic Bilirubin Concentrations on Apoptosis

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    Author
    Njonkou Tchoquessi, Rosy Linda
    Advisor
    Bearer, Cynthia F.
    Date
    2019
    Type
    dissertation
    
    Metadata
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    Abstract
    Background: The mechanisms of bilirubin neurotoxicity are poorly understood. We hypothesize that free bilirubin at concentrations found in preterm infants increases neuronal apoptosis. To test this hypothesis, we measured the expression of pro-apoptotic proteins in primary cultures of cerebellar granule neurons exposed to bilirubin using quantitative immunoblotting techniques. Methods: Cerebellar granule neurons were plated on poly-L-Lysine overnight, then treated with human serum albumin combined with bilirubin to produce physiologic concentrations of free bilirubin. Staurosporine was added to some cultures as a positive control. Cell lysates were prepared, and cleaved caspase 3 expression was determined using quantitative immunoblotting techniques. Results: Addition of physiologic concentrations of free bilirubin to cerebellar granule neurons increased the expression of cleaved caspase 3 in a dose dependent manner at 24 hours. Conclusions: Physiologic concentrations of free bilirubin induces apoptosis in cultured rat cerebellar granule neurons.
    Description
    2019
    Molecular Medicine
    University of Maryland, Baltimore
    M.S.
    Keyword
    Molecular biology
    free bilirubin
    kernicterus spectrum disorders
    Apoptosis
    Jaundice, Neonatal
    Identifier to cite or link to this item
    http://hdl.handle.net/10713/11488
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    Theses and Dissertations School of Medicine
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