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dc.contributor.authorSengyee, S.
dc.contributor.authorYoon, S.H.
dc.contributor.authorWest, T.E.
dc.date.accessioned2019-12-04T15:55:05Z
dc.date.available2019-12-04T15:55:05Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85075222273&doi=10.1128%2fIAI.00692-19&partnerID=40&md5=aa9ece7785b5fca3fdc0d78d1a496a4c
dc.identifier.urihttp://hdl.handle.net/10713/11462
dc.description.abstractLipopolysaccharides (LPSs) of Gram-negative bacteria comprise lipid A, core, and O-polysaccharide (OPS) components. Studies have demonstrated that LPSs isolated from the pathogenic species Burkholderia pseudomallei and Burkholderia mallei and from less-pathogenic species, such as Burkholderia thailandensis, are potent immune stimulators. The LPS structure of B. pseudomallei, the causative agent of melioidosis, is highly conserved in isolates from Thailand; however, the LPSs isolated from other, related species have not been characterized to enable understanding of their immune recognition and antigenicities. Here, we describe the structural and immunological characteristics of the LPSs isolated from eight Burkholderia species and compare those for B. pseudomallei to those for the other seven species. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), gas chromatography (GC), SDS-PAGE, Toll-like receptor 4 (TLR4) stimulation, and immunoblot analysis were performed on these Burkholderia species. MALDI-TOF profiles demonstrated that Burkholderia lipid A contains predominantly penta-acylated species modified with 4-amino-4-deoxy-arabinose residues at both terminal phosphate groups. The lipid A could be differentiated based on mass differences at m/z 1,511, 1,642, 1,773, and 1,926 and on fatty acid composition. LPSs of all species induced TLR4-dependent NF-?B responses; however, while SDS-PAGE analysis showed similar LPS ladder patterns for B. pseudomallei, B. thailandensis, and B. mallei, these patterns differed from those of other Burkholderia species. Interestingly, immunoblot analysis demonstrated that melioidosis patient sera cross-reacted with OPSs of other Burkholderia species. These findings can be used to better understand the characteristics of LPS in Burkholderia species, and they have implications for serological diagnostics based on the detection of antibodies to OPS. Copyright 2019 Sengyee et al.en_US
dc.description.urihttps://doi.org/10.1128/IAI.00692-19en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.ispartofInfection and immunity
dc.subjectantibodyen_US
dc.subjectbacterial infectionen_US
dc.subjectBurkholderiaen_US
dc.subjectdiagnosticsen_US
dc.subjectinnate immunityen_US
dc.subjectlipopolysaccharideen_US
dc.subjectLPS structureen_US
dc.subjectmelioidosisen_US
dc.subjectO-polysaccharideen_US
dc.subjectserologyen_US
dc.subjectTLR4en_US
dc.titleLipopolysaccharides from Different Burkholderia Species with Different Lipid A Structures Induce Toll-Like Receptor 4 Activation and React with Melioidosis Patient Seraen_US
dc.typeArticleen_US
dc.identifier.doi10.1128/IAI.00692-19
dc.identifier.pmid31548320


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