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dc.contributor.authorFilcek, K.
dc.contributor.authorVielfort, K.
dc.contributor.authorBavoil, P.M.
dc.date.accessioned2019-11-20T15:51:42Z
dc.date.available2019-11-20T15:51:42Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85074644319&doi=10.1371%2fjournal.pone.0224324&partnerID=40&md5=981a50310312d951056a9f79e91c78dd
dc.identifier.urihttp://hdl.handle.net/10713/11429
dc.description.abstractThe ability to introduce targeted genetic modifications in microbial genomes has revolutionized our ability to study the role and mode of action of individual bacterial virulence factors. Although the fastidious lifestyle of obligate intracellular bacterial pathogens poses a technical challenge to such manipulations, the last decade has produced significant advances in our ability to conduct molecular genetic analysis in Chlamydia trachomatis, a major bacterial agent of infertility and blindness. Similar approaches have not been established for the closely related veterinary Chlamydia spp., which cause significant economic damage, as well as rare but potentially life-threatening infections in humans. Here we demonstrate the feasibility of conducting site-specific mutagenesis for disrupting virulence genes in C. caviae, an agent of guinea pig inclusion conjunctivitis that was recently identified as a zoonotic agent in cases of severe community-acquired pneumonia. Using this approach, we generated C. caviae mutants deficient for the secreted effector proteins IncA and SinC. We demonstrate that C. caviae IncA plays a role in mediating fusion of the bacteria-containing vacuoles inhabited by C. caviae. Moreover, using a chicken embryo infection model, we provide first evidence for a role of SinC in C. caviae virulence in vivo. Copyright 2019 Filcek et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.sponsorshipThis work was supported by grants from the National Institutes of Health (RHV: R01AI100759, PMB: STI Cooperative Research Center U19 AI 084044), the European Union's Seventh Framework Program (BSS: PIOF-GA-2013-626116), the Swedish Research Council (BSS: project 2018-02286, MIMS - The Swedish EMBL node for Molecular Medicine: project 2016-06598), and the Kempe foundation (SM: fellowship JCK-1834).en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0224324en_US
dc.language.isoen_USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONE
dc.subjectChlamydia caviaeen_US
dc.subjectmolecular genetic analysisen_US
dc.subject.meshMutagenesis, Insertionalen_US
dc.subject.meshMutagenesis, Site-Directeden_US
dc.subject.meshGenome, Microbialen_US
dc.subject.meshVirulence Factorsen_US
dc.subject.meshChlamydia trachomatisen_US
dc.titleInsertional mutagenesis in the zoonotic pathogen Chlamydia caviaeen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0224324
dc.identifier.pmid31697687


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