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dc.contributor.authorChandra, R.
dc.contributor.authorCalarco, C.A.
dc.contributor.authorLobo, M.K.
dc.date.accessioned2019-11-06T15:55:50Z
dc.date.available2019-11-06T15:55:50Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85070497902&doi=10.1002%2fjnr.24511&partnerID=40&md5=555ab9d0ce68e57dc16f7abe91b74e61
dc.identifier.urihttp://hdl.handle.net/10713/11381
dc.description.abstractThe two striatal projection neuron subtypes (medium spiny neurons- MSNs), those enriched in dopamine receptor 1 versus 2 (D1-MSNs and D2-MSNs), display dichotomous properties at the level of the transcriptome, projections, morphology, and electrophysiology. Recent work illustrates dichotomous mitochondrial length in NAc MSN subtype dendrites after cocaine self-administration, with a shift toward smaller mitochondria, due to enhanced fission, occurring in D1-MSN dendrites and a shift toward larger mitochondria in D2-MSN dendrites. However, to date there has been no comparison of mitochondrial morphological properties between MSN subtypes. In this study, we examine mitochondrial morphology in NAc D1-MSNs versus D2-MSNs. We observe an increase in the frequency of smaller length mitochondria in D2-MSN dendrites relative to D1-MSN dendrites, while D1-MSN dendrites display an increase in larger length mitochondria. The differences in mitochondrial length occur in both NAc core and shell, although to a greater extent in NAc core. Finally, we demonstrate that the mitochondrial fusion molecule, Opa1, is differentially expressed in NAc MSN subtypes, with D1-MSNs displaying higher expression of Opa1 ribosome-associated mRNA. The difference in Opa1 levels may account for the bias toward enhanced smaller mitochondria in D2-MSNs and enhanced larger mitochondria in D1-MSNs. Collectively, our study demonstrates differential mitochondrial size and a potential molecular mediator of these mitochondrial differences in NAc MSN subtypes.en_US
dc.description.sponsorshipThis work is supported by R01DA038613 to M.K.L., the Brain and Behavior Research Foundation (NARSAD Young Investigator grant number 23621, P&S Fund) to R.C., and T32DK098107 to C.A.C.en_US
dc.description.urihttps://doi.org/10.1002/jnr.24511en_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley and Sons Inc.en_US
dc.relation.ispartofJournal of Neuroscience Research
dc.subjectmedium spiny neuronsen_US
dc.subjectmitochondriaen_US
dc.subjectnucleus accumbensen_US
dc.subjectOpa1en_US
dc.subjectRRID:AB_10000240en_US
dc.subjectRRID:AB_2340375en_US
dc.subjectRRID:AB_291262en_US
dc.subjectRRID:IMSR_JAX:029977en_US
dc.subjectRRID:MGI:5304499en_US
dc.subjectRRID:MGI:5528384en_US
dc.subjectRRID:SCR_007370en_US
dc.titleDifferential mitochondrial morphology in ventral striatal projection neuron subtypesen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/jnr.24511
dc.identifier.pmid31392754


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