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    Differential mitochondrial morphology in ventral striatal projection neuron subtypes

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    Author
    Chandra, R.
    Calarco, C.A.
    Lobo, M.K.
    Date
    2019
    Journal
    Journal of Neuroscience Research
    Publisher
    John Wiley and Sons Inc.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1002/jnr.24511
    Abstract
    The two striatal projection neuron subtypes (medium spiny neurons- MSNs), those enriched in dopamine receptor 1 versus 2 (D1-MSNs and D2-MSNs), display dichotomous properties at the level of the transcriptome, projections, morphology, and electrophysiology. Recent work illustrates dichotomous mitochondrial length in NAc MSN subtype dendrites after cocaine self-administration, with a shift toward smaller mitochondria, due to enhanced fission, occurring in D1-MSN dendrites and a shift toward larger mitochondria in D2-MSN dendrites. However, to date there has been no comparison of mitochondrial morphological properties between MSN subtypes. In this study, we examine mitochondrial morphology in NAc D1-MSNs versus D2-MSNs. We observe an increase in the frequency of smaller length mitochondria in D2-MSN dendrites relative to D1-MSN dendrites, while D1-MSN dendrites display an increase in larger length mitochondria. The differences in mitochondrial length occur in both NAc core and shell, although to a greater extent in NAc core. Finally, we demonstrate that the mitochondrial fusion molecule, Opa1, is differentially expressed in NAc MSN subtypes, with D1-MSNs displaying higher expression of Opa1 ribosome-associated mRNA. The difference in Opa1 levels may account for the bias toward enhanced smaller mitochondria in D2-MSNs and enhanced larger mitochondria in D1-MSNs. Collectively, our study demonstrates differential mitochondrial size and a potential molecular mediator of these mitochondrial differences in NAc MSN subtypes.
    Sponsors
    This work is supported by R01DA038613 to M.K.L., the Brain and Behavior Research Foundation (NARSAD Young Investigator grant number 23621, P&S Fund) to R.C., and T32DK098107 to C.A.C.
    Keyword
    medium spiny neurons
    mitochondria
    nucleus accumbens
    Opa1
    RRID:AB_10000240
    RRID:AB_2340375
    RRID:AB_291262
    RRID:IMSR_JAX:029977
    RRID:MGI:5304499
    RRID:MGI:5528384
    RRID:SCR_007370
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    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85070497902&doi=10.1002%2fjnr.24511&partnerID=40&md5=555ab9d0ce68e57dc16f7abe91b74e61; http://hdl.handle.net/10713/11381
    ae974a485f413a2113503eed53cd6c53
    10.1002/jnr.24511
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