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    Promoter architecture and transcriptional regulation of musculoskeletal embryonic nuclear protein 1b (mustn1b) gene in zebrafish

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    Author
    Suarez-Bregua, P.
    Chien, C.-J.
    Megias, M.
    Date
    2017
    Journal
    Developmental Dynamics
    Publisher
    John Wiley and Sons Inc.
    Type
    Article
    
    Metadata
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    See at
    https://doi.org/10.1002/dvdy.24591
    Abstract
    Background: Mustn1 is a specific musculoskeletal protein that plays a critical role in myogenesis and chondrogenesis in vertebrates. Whole-mount in situ hybridization revealed that mustn1b mRNAs are specifically expressed in skeletal and cardiac muscles in Zebrafish embryos. However, the precise function and the regulatory elements required for its muscle-specific expression are largely unknown. Results: The purpose of this study was to explore and uncover the target genomic regions that regulate mustn1b gene expression by in vivo functional characterization of the mustn1b promoter. We report here stable expression analyses of eGFP from fluorescent transgenic reporter Zebrafish line containing a 0.8kb_mustn1b-Tol2-eGFP construct. eGFP expression was specifically found in the skeletal and cardiac muscle tissues. We show that reporter Zebrafish lines generated replicate the endogenous mustn1b expression pattern in early Zebrafish embryos. Specific site directed-mutagenesis analysis revealed that promoter activity resides in two annotated genomic regulatory regions, each one corresponding to a specific functional transcription factor binding site. Conclusions: Our data indicate that mustn1b is specifically expressed in skeletal and cardiac muscle tissues and its muscle specificity is controlled by the 0.2-kb promoter and flanking sequences and in vivo regulated by the action of two sequence-specific families of transcription factors. Developmental Dynamics 246:992-1000, 2017.
    Sponsors
    This work was funded by the Spanish Science and Innovation Ministry grants ALG2011-23581 and AGL2014-52473R (to J.R.). P. Suarez-Bregua was supported by a Campus do Mar PhD grant, Xunta de Galicia.
    Keyword
    cardiac muscle
    mef2
    muscle-specificity
    myod
    skeletal muscle
    transgenic Zebrafish
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85033778354&doi=10.1002%2fdvdy.24591&partnerID=40&md5=e0de9376cd46eeb23fc629f2522edfe3; http://hdl.handle.net/10713/11333
    ae974a485f413a2113503eed53cd6c53
    10.1002/dvdy.24591
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