Modulation of Staphylococcus aureus response to antimicrobials by the Candida albicans quorum sensing molecule farnesol
JournalAntimicrobial Agents and Chemotherapy
PublisherAmerican Society for Microbiology
MetadataShow full item record
AbstractIn microbial biofilms, microorganisms utilize secreted signaling chemical molecules to coordinate their collective behavior. Farnesol is a quorum sensing molecule secreted by the fungal species Candida albicans and shown to play a central physiological role during fungal biofilm growth. Our pervious in vitro and in vivo studies characterized an intricate interaction between C. albicans and the bacterial pathogen Staphylococcus aureus, as these species coexist in biofilm. In this study, we aimed to investigate the impact of farnesol on S. aureus survival, biofilm formation, and response to antimicrobials. The results demonstrated that in the presence of exogenously supplemented farnesol or farnesol secreted by C. albicans in biofilm, S. aureus exhibited significantly enhanced tolerance to antimicrobials. By using gene expression studies, S. aureus mutant strains, and chemical inhibitors, the mechanism for the enhanced tolerance was attributed to upregulation of drug efflux pumps. Importantly, we showed that sequential exposure of S. aureus to farnesol generated a phenotype of high resistance to antimicrobials. Based on the presence of intracellular reactive oxygen species upon farnesol exposure, we hypothesize that antimicrobial tolerance in S. aureus may be mediated by farnesol-induced oxidative stress triggering the upregulation of efflux pumps, as part of a general stress response system. Hence, in mixed biofilms, C. albicans may influence the pathogenicity of S. aureus through acquisition of a drug-tolerant phenotype, with important therapeutic implications. Understanding interspecies signaling in polymicrobial biofilms and the specific drug resistance responses to secreted molecules may lead to the identification of novel targets for drug development.
SponsorsThis work was supported by the National Institute of Allergy and Infectious Diseases of the NIH under award number R01AI130170 to Mary Ann Jabra-Rizk and by the Interuniversity Attraction Poles Programme initiated by the Belgian Science Policy Office to Patrick Van Dijck and Mary Ann Jabra-Rizk. This work utilized an EM sample preparation instrument that was purchased with funding from a National Institutes of Health SIG grant (1S10RR26870-1) awarded to the University of Maryland, Baltimore.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85035056678&doi=10.1128%2fAAC.01573-17&partnerID=40&md5=c0a8c2cfb07328ccb846c814a91f411c; http://hdl.handle.net/10713/11297
- <i>Candida albicans</i> quorum-sensing molecule farnesol modulates staphyloxanthin production and activates the thiol-based oxidative-stress response in <i>Staphylococcus aureus</i>.
- Authors: Vila T, Kong EF, Ibrahim A, Piepenbrink K, Shetty AC, McCracken C, Bruno V, Jabra-Rizk MA
- Issue date: 2019 Dec
- Prostaglandin E2 from Candida albicans Stimulates the Growth of Staphylococcus aureus in Mixed Biofilms.
- Authors: Krause J, Geginat G, Tammer I
- Issue date: 2015
- Commensal Protection of Staphylococcus aureus against Antimicrobials by Candida albicans Biofilm Matrix.
- Authors: Kong EF, Tsui C, Kucharíková S, Andes D, Van Dijck P, Jabra-Rizk MA
- Issue date: 2016 Oct 11
- Confocal laser scanning microscopy analysis of S. epidermidis biofilms exposed to farnesol, vancomycin and rifampicin.
- Authors: Cerca N, Gomes F, Pereira S, Teixeira P, Oliveira R
- Issue date: 2012 May 16
- Impact of a Cross-Kingdom Signaling Molecule of Candida albicans on Acinetobacter baumannii Physiology.
- Authors: Kostoulias X, Murray GL, Cerqueira GM, Kong JB, Bantun F, Mylonakis E, Khoo CA, Peleg AY
- Issue date: 2016 Jan