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dc.contributor.authorHolt, J.E.
dc.contributor.authorHouston, A.
dc.contributor.authorAdams, C.
dc.date.accessioned2019-11-01T12:49:35Z
dc.date.available2019-11-01T12:49:35Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85028657036&doi=10.1093%2ffemspd%2fftx052&partnerID=40&md5=9d6627c578b3645ad16b45e514216968
dc.identifier.urihttp://hdl.handle.net/10713/11288
dc.description.abstractBiofilms are formed by communities of microorganisms living in a self-produced extracellular polymeric matrix attached to a surface. When living in a biofilm microorganisms change phenotype and thus are less susceptible to antibiotic treatment and biofilm infections can become severe. The aim of this study was to determine if the presence of multikingdom microorganisms alters the virulence of a biofilm infection in a host organism. The coexistence of Candida albicans and Staphylococcus epidermidis in biofilm was examined in the nematode model Caenorhabditis elegans. It was evaluated if the hyphal form of C. albicans and extracellular polymeric substances (EPS) formed by S. epidermidis increases biofilm virulence. Survival assays were performed, where C. elegans nematodes were exposed to S. epidermidis and C. albicans. Single inoculation assays showed a decreased survival rate after 2 days following exposure, while dual inoculation assays showed that a clinical S. epidermidis strain together with C. albicans significantly increased the virulence and decreased nematode survival. EPS seem to interfere with the bacterial attachment to hyphae, since the EPS overproducing S. epidermidis strain was most virulent. The clinical S. epidermidis paired with C. albicans led to a severe infection in the nematodes resulting in reduced survival.en_US
dc.description.sponsorshipThis work was funded through the National Science Foundation's STEP Program, the Successful Engineering Education and Development Support (SEEDS) Program, where fellowships during 2016-17 were awarded to Jillian Holt. We also want to thank The Caenorhabditis Genetics Center (CGC) that is supported by the National Institutes of Health - Office of Research Infrastructure Programs ( P40 OD010440).en_US
dc.description.urihttps://doi.org/10.1093/femspd/ftx052en_US
dc.language.isoen_USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofPathogens and Disease
dc.subjectBiofilmen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectCandida Albicansen_US
dc.subjectInfectionen_US
dc.subjectStaphylococcus epidermidisen_US
dc.subjectVirulenceen_US
dc.titleRole of extracellular polymeric substances in polymicrobial biofilm infections of Staphylococcus epidermidis and Candida albicans modelled in the nematode Caenorhabditis elegansen_US
dc.typeArticleen_US
dc.identifier.doi10.1093/femspd/ftx052
dc.identifier.pmid28475673


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