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    Deep sequencing of the TCR‐β repertoire of human forkhead box protein 3 (FoxP3)+and foxp3-T cells suggests that they are completely distinct and non-overlapping

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    Author
    Golding, A.
    Darko, S.
    Wylie, W.H.
    Date
    2017
    Journal
    Clinical and Experimental Immunology
    Publisher
    Blackwell Publishing Ltd
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1111/cei.12904
    Abstract
    Maintenance of peripheral tolerance requires a balance between autoreactive conventional T cells (Tconv) and thymically derived forkhead box protein 3 (FoxP3)1 regulatory T cells (tTregs). Considerable controversy exists regarding the similarities/differences in T cell receptor (TCR) repertoires expressed by Tconvand tTregs. We generated highly purified populations of human adult and cord blood Tconvand tTregsbased on the differential expression of CD25 and CD127. The purity of the sorted populations was validated by intracellular staining for FoxP3 and Helios. We also purified an overlap group of CD4 T cells from adult donors to ensure that considerable numbers of shared clonotypes could be detected when present. We used deep sequencing of entire TCR-b CDR3 sequences to analyse the TCR repertoire of Tconvand tTregs. Our studies suggest that both neonatal and adult human Tconvand tTregcells are, in fact, entirely distinct CD4 T cell lineages. Copyright 2016 British Society for Immunology, Clinical and Experimental Immunology.
    Sponsors
    The NIAID/LI Sorting Facility is acknowledged. This work was supported by funds from the Intramural Research program of the National Institute of Allergy and Infectious Diseases. A. G. is a VA CDA awardee at the Baltimore VA/ VAMHCS and acknowledges funding from the VA Career Development Award (1K2 CX000649).
    Keyword
    Deep sequencing
    Regulatory T cells
    T cells repertoire
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85008462050&doi=10.1111%2fcei.12904&partnerID=40&md5=70b0dd92fe0031680ceebdc98fcb0e4a; http://hdl.handle.net/10713/11278
    ae974a485f413a2113503eed53cd6c53
    10.1111/cei.12904
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    UMB Open Access Articles 2017

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