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dc.contributor.authorWang, L.
dc.contributor.authorMa, X.
dc.contributor.authorXu, X.
dc.date.accessioned2019-11-01T12:49:32Z
dc.date.available2019-11-01T12:49:32Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85018965799&doi=10.1038%2fs41598-017-00823-3&partnerID=40&md5=d512ba1e97f65cde94f135f1e1c5376a
dc.identifier.urihttp://hdl.handle.net/10713/11258
dc.description.abstractLong intergenic noncoding RNAs (lincRNAs) are increasingly recognized as potential key regulators of heart development and related diseases, but their identities and functions remain elusive. In this study, we sought to identify and characterize the cardiac lincRNA transcriptome in the experimentally accessible zebrafish model by integrating bioinformatics analysis with experimental validation. By conducting genome-wide RNA sequencing profiling of zebrafish embryonic hearts, adult hearts, and adult muscle, we generated a high-confidence set of 813 cardiac lincRNA transcripts, 423 of which are novel. Among these lincRNAs, 564 are expressed in the embryonic heart, and 730 are expressed in the adult heart, including 2 novel lincRNAs, TCONS-00000891 and TCONS-00028686, which exhibit cardiac-enriched expression patterns in adult heart. Using a method similar to a fetal gene program, we identified 51 lincRNAs with differential expression patterns between embryonic and adult hearts, among which TCONS-00009015 responded to doxorubicin-induced cardiac stress. In summary, our genome-wide systematic identification and characterization of cardiac lincRNAs lays the foundation for future studies in this vertebrate model to elucidate crucial roles for cardiac lincRNAs during heart development and cardiac diseases. Copyright 2017 The Author(s).en_US
dc.description.sponsorshipThis work was supported by the National Institutes of Health (P30CA134274 to the University of Maryland Baltimore; HL81753 and HL107304 to X.X.) and the Mayo Foundation (to X.X.). Funding for open access charge: National Institutes of Health.en_US
dc.description.urihttps://doi.org/10.1038/s41598-017-00823-3en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofScientific Reports
dc.subjectlong intergenic noncoding RNAsen_US
dc.subjectcardiovascular geneticsen_US
dc.subjectgenome informaticsen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshHeart--embryologyen_US
dc.subject.meshHeart--growth & developmenten_US
dc.subject.meshRNA, Untranslated--geneticsen_US
dc.subject.meshZebrafishen_US
dc.titleSystematic identification and characterization of cardiac long intergenic noncoding RNAs in zebrafishen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-017-00823-3
dc.identifier.pmid28455512


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