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dc.contributor.authorNikolskaia, O.V.
dc.contributor.authorThekisoe, O.M.
dc.contributor.authorStephen, Dumler, J.
dc.date.accessioned2019-10-08T19:43:54Z
dc.date.available2019-10-08T19:43:54Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85014571151&doi=10.4269%2fajtmh.15-0288&partnerID=40&md5=1b6675f04ba379bf53c7a4711c9fc2f3
dc.identifier.urihttp://hdl.handle.net/10713/11143
dc.description.abstractThe loop-mediated isothermal amplification (LAMP) assay with its advantages of cost effectiveness, rapidity, and simplicity, has evolved as a sensitive and specific method for the detection of African trypanosomes. Highly sensitive LAMP reactions specific for Trypanosoma brucei rhodesiense or that recognize but do not discriminate between Trypanosoma brucei brucei, T. b. rhodesiense, Trypanosoma brucei gambiense, and Trypanosoma evansi have been developed. A sensitive LAMP assay targeting the T. b. gambiense 5.8S ribosomal RNA internal transcribed spacer 2 (5.8SITS2) gene is also available but this assay does not target binding sites that span the CCCA (C3A) (557-560 bps) insertion site that further differentiates T. b. gambiense from T. b. brucei. Here we describe 5.8S-ITS2-targeted LAMP assay that fit these criteria. The LAMP primer sets containing the T. b. gambiense-specific C3A tetranucleotide at the start of the outer forward primer sequences showed high specificity and sensitivity down to at least 0.1 fg T. b. gambiense genomic DNA.en_US
dc.description.sponsorshipThis research was supported in part by grants from the National Institutes of Health (5R01AI082695 and 1R21AI079282).en_US
dc.description.urihttps://doi.org/10.4269/ajtmh.15-0288en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society of Tropical Medicine and Hygieneen_US
dc.relation.ispartofAmerican Journal of Tropical Medicine and Hygiene
dc.subjectloop-mediated isothermal amplificationen_US
dc.subjectLAMPen_US
dc.subject.meshGenes, Protozoan--geneticsen_US
dc.subject.meshRNA, Ribosomal, 5.8S--geneticsen_US
dc.subject.meshTrypanosoma brucei gambiense--geneticsen_US
dc.subject.meshTrypanosomiasis, African--diagnosisen_US
dc.titleLoop-Mediated isothermal amplification for detection of the 5.8s ribosomal ribonucleic acid internal transcribed spacer 2 gene found in trypanosoma brucei gambienseen_US
dc.typeArticleen_US
dc.identifier.doi10.4269/ajtmh.15-0288
dc.identifier.pmid27273643


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