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dc.contributor.authorMarsh, J.W.
dc.contributor.authorHumphrys, M.S.
dc.contributor.authorMyers, G.S.A.
dc.date.accessioned2019-10-08T19:43:49Z
dc.date.available2019-10-08T19:43:49Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85029677487&doi=10.3389%2ffmicb.2017.01830&partnerID=40&md5=aef3f1e9a8a9e18ce7b68c0b87b76c89
dc.identifier.urihttp://hdl.handle.net/10713/11079
dc.description.abstractDual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the same biological sample, dual RNA-Seq can provide unique insight into bacterial infection processes and reciprocal host responses at once. However, the difficulties involved in handling both prokaryotic and eukaryotic material require distinct, optimized procedures. We previously developed and applied dual RNA-Seq to measure prokaryotic and eukaryotic expression profiles of human cells infected with bacteria, using in vitro Chlamydia-infected epithelial cells as proof of principle. Here we provide a detailed laboratory protocol for in vitro dual RNA-Seq that is readily adaptable to any host-bacteria system of interest. Copyright 2017 Marsh, Humphrys and Myers.en_US
dc.description.urihttps://doi.org/10.3389/fmicb.2017.01830en_US
dc.language.isoen_USen_US
dc.publisherFrontiers Media S.A.en_US
dc.relation.ispartofFrontiers in Microbiology
dc.subjectBacteriaen_US
dc.subjectChlamydiaen_US
dc.subjectDual RNA-Seqen_US
dc.subjectHosten_US
dc.subjectProtocolen_US
dc.titleA laboratory methodology for dual RNA-sequencing of bacteria and their host cells in vitroen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2017.01830


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