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    A laboratory methodology for dual RNA-sequencing of bacteria and their host cells in vitro

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    Author
    Marsh, J.W.
    Humphrys, M.S.
    Myers, G.S.A.
    Date
    2017
    Journal
    Frontiers in Microbiology
    Publisher
    Frontiers Media S.A.
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.3389/fmicb.2017.01830
    Abstract
    Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the same biological sample, dual RNA-Seq can provide unique insight into bacterial infection processes and reciprocal host responses at once. However, the difficulties involved in handling both prokaryotic and eukaryotic material require distinct, optimized procedures. We previously developed and applied dual RNA-Seq to measure prokaryotic and eukaryotic expression profiles of human cells infected with bacteria, using in vitro Chlamydia-infected epithelial cells as proof of principle. Here we provide a detailed laboratory protocol for in vitro dual RNA-Seq that is readily adaptable to any host-bacteria system of interest. Copyright 2017 Marsh, Humphrys and Myers.
    Keyword
    Bacteria
    Chlamydia
    Dual RNA-Seq
    Host
    Protocol
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029677487&doi=10.3389%2ffmicb.2017.01830&partnerID=40&md5=aef3f1e9a8a9e18ce7b68c0b87b76c89; http://hdl.handle.net/10713/11079
    ae974a485f413a2113503eed53cd6c53
    10.3389/fmicb.2017.01830
    Scopus Count
    Collections
    UMB Open Access Articles 2017

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