A laboratory methodology for dual RNA-sequencing of bacteria and their host cells in vitro
Date
2017Journal
Frontiers in MicrobiologyPublisher
Frontiers Media S.A.Type
Article
Metadata
Show full item recordAbstract
Dual RNA-Sequencing leverages established next-generation sequencing (NGS)-enabled RNA-Seq approaches to measure genome-wide transcriptional changes of both an infecting bacteria and host cells. By simultaneously investigating both organisms from the same biological sample, dual RNA-Seq can provide unique insight into bacterial infection processes and reciprocal host responses at once. However, the difficulties involved in handling both prokaryotic and eukaryotic material require distinct, optimized procedures. We previously developed and applied dual RNA-Seq to measure prokaryotic and eukaryotic expression profiles of human cells infected with bacteria, using in vitro Chlamydia-infected epithelial cells as proof of principle. Here we provide a detailed laboratory protocol for in vitro dual RNA-Seq that is readily adaptable to any host-bacteria system of interest. Copyright 2017 Marsh, Humphrys and Myers.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85029677487&doi=10.3389%2ffmicb.2017.01830&partnerID=40&md5=aef3f1e9a8a9e18ce7b68c0b87b76c89; http://hdl.handle.net/10713/11079ae974a485f413a2113503eed53cd6c53
10.3389/fmicb.2017.01830