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dc.contributor.authorHotopp, J.C.D.
dc.contributor.authorSlatko, B.E.
dc.contributor.authorFoster, J.M.
dc.date.accessioned2019-10-08T19:43:48Z
dc.date.available2019-10-08T19:43:48Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85018268181&doi=10.1038%2fs41598-017-00814-4&partnerID=40&md5=fd934a4087ce516fa729c91ffa1729ad
dc.identifier.urihttp://hdl.handle.net/10713/11062
dc.description.abstractLateral gene transfer (LGT) from microbial symbionts to invertebrate animals is described at an increasing rate, particularly between Wolbachia endosymbionts and their diverse invertebrate hosts. We sought to assess the use of a capture system to cost-effectively sequence such LGT from the host genome. The sequencing depth of Illumina paired end data obtained with a Wolbachia capture system correlated well with that for an Illumina paired end data set used to detect LGT in Wolbachia-depleted B. malayi (p-value: <2e-16). Using a sequencing depth threshold of two or three standard deviations above the mean, 96.9% or 96.7% of positions, respectively, are predicted in the same manner between the two datasets, with 24.7% or 42.5% of the known 49.0 kbp of LGT sequence predicted correctly, respectively. Prior qPCR results for nuwts showed similar correlations for both datasets supporting our conclusion that oligonucleotide-based capture methods can be used to obtain sequences from Wolbachia-host LGT. However, at least 121 positions had a minority of the reads supporting the endosymbiont reference base call using the capture data, illustrating that sequence reads from endosymbiont-host LGTs can confound endosymbiont genome projects, erroneously altering the called consensus genome, a problem that is irrespective to the sequencing technology or platform. Copyright 2017 The Author(s).en_US
dc.description.sponsorshipThis work was funded by the National Institutes of Health through the NIH Director's New Innovator Award Program (1-DP2-OD007372), National Institute of Allergy and Infectious Diseases (U19AI110820), and internal funding from New England Biolabs, Inc.en_US
dc.description.urihttps://doi.org/10.1038/s41598-017-00814-4en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofScientific Reports
dc.subjectendosymbionten_US
dc.subjectIllumina paired-end dataen_US
dc.subject.meshGene Transfer, Horizontalen_US
dc.subject.meshInvertebratesen_US
dc.subject.meshWolbachiaen_US
dc.subject.meshGenomeen_US
dc.subject.meshHigh-Throughput Nucleotide Sequencingen_US
dc.subject.meshReal-Time Polymerase Chain Reactionen_US
dc.subject.meshOligonucleotidesen_US
dc.titleTargeted Enrichment and Sequencing of Recent Endosymbiont-Host Lateral Gene Transfersen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-017-00814-4
dc.identifier.pmid28405008


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