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dc.contributor.authorLi, S.
dc.contributor.authorGeddes, C.D.
dc.contributor.authorStine, O. Colinen_US
dc.date.accessioned2019-10-08T13:01:10Z
dc.date.available2019-10-08T13:01:10Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85072724148&doi=10.1371%2fjournal.pone.0223008&partnerID=40&md5=43b4c6aecf92956cf2ac971893f471d5
dc.identifier.urihttp://hdl.handle.net/10713/11057
dc.description.abstractNucleases are enzymes that can degrade genomic DNA and RNA that decrease the accuracy of quantitative measures of those nucleic acids. Here, we study conventional heating, standard microwave irradiation, and Lyse-It, a microwave-based lysing technology, for the potential to fragment and inactivate DNA and RNA endonucleases. Lyse-It employs the use of highly focused microwave irradiation to the sample ultimately fragmenting and inactivating RNase A, RNase B, and DNase I. Nuclease size and fragmentation were determined visually and quantitatively by SDS polyacrylamide gel electrophoresis and the mini-gel Agilent 2100 Bioanalyzer system, with a weighted size calculated to depict the wide range of nuclease fragmentation. Enzyme activity assays were conducted, and the rates were calculated to determine the effect of various lysing conditions on each of the nucleases. The results shown in this paper clearly demonstrate that Lyse-It is a rapid and highly efficient way to degrade and inactivate nucleases so that nucleic acids can be retained for downstream detection. Copyright 2019 Santaus et al.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0223008en_US
dc.language.isoen-USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONE
dc.subjectLyse-Iten_US
dc.subject.meshEndonucleases--antagonists & inhibitorsen_US
dc.titleEffects of Lyse-It on endonuclease fragmentation, function and activityen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0223008
dc.identifier.pmid31568482


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