Microparticle and interleukin-1β production with human simulated compressed air diving
Date
2019Journal
Scientific reportsPublisher
Nature Publishing GroupType
Article
Metadata
Show full item recordAbstract
Production of blood-borne microparticles (MPs), 0.1–1 µm diameter vesicles, and interleukin (IL)-1β in response to high pressure is reported in lab animals and associated with pathological changes. It is unknown whether the responses occur in humans, and whether they are due to exposure to high pressure or to the process of decompression. Blood from research subjects exposed in hyperbaric chambers to air pressure equal to 18 meters of sea water (msw) for 60 minutes or 30 msw for 35 minutes were obtained prior to and during compression and 2 hours post-decompression. MPs and intra-particle IL-1β elevations occurred while at pressure in both groups. At 18 msw (n = 15) MPs increased by 1.8-fold, and IL-1β by 7.0-fold (p < 0.05, repeated measures ANOVA on ranks). At 30 msw (n = 16) MPs increased by 2.5-fold, and IL-1β by 4.6-fold (p < 0.05), and elevations persisted after decompression with MPs elevated by 2.0-fold, and IL-1β by 6.0-fold (p < 0.05). Whereas neutrophils incubated in ambient air pressure for up to 3 hours ex vivo did not generate MPs, those exposed to air pressure at 180 kPa for 1 hour generated 1.4 ± 0.1 MPs/cell (n = 8, p < 0.05 versus ambient air), and 1.7 ± 0.1 MPs/cell (p < 0.05 versus ambient air) when exposed to 300 kPa for 35 minutes. At both pressures IL-1β concentration tripled (p < 0.05 versus ambient air) during pressure exposure and increased 6-fold (p < 0.05 versus ambient air) over 2 hours post-decompression. Platelets also generated MPs but at a rate about 1/100 that seen with neutrophils. We conclude that production of MPs containing elevated concentrations of IL-1β occur in humans during exposure to high gas pressures, more so than as a response to decompression. While these events may pose adverse health threats, their contribution to decompression sickness development requires further study.Sponsors
Tis project was supported by the Canadian Forces Surgeon General Health Research Program, Grant N00014-16-1-2868 from the Ofce of Naval Research and an unrestricted grant from the National Foundation of Emergency Medicine.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072283974&doi=10.1038%2fs41598-019-49924-1&partnerID=40&md5=a0066e812c60877ed2d109f9a62b071a; http://hdl.handle.net/10713/11023ae974a485f413a2113503eed53cd6c53
10.1038/s41598-019-49924-1
Scopus Count
Collections
Related articles
- Microparticle-induced vascular injury in mice following decompression is inhibited by hyperbaric oxygen: effects on microparticles and interleukin-1β.
- Authors: Thom SR, Bhopale VM, Yang M
- Issue date: 2019 Apr 1
- Association of microparticles and neutrophil activation with decompression sickness.
- Authors: Thom SR, Bennett M, Banham ND, Chin W, Blake DF, Rosen A, Pollock NW, Madden D, Barak O, Marroni A, Balestra C, Germonpre P, Pieri M, Cialoni D, Le PN, Logue C, Lambert D, Hardy KR, Sward D, Yang M, Bhopale VB, Dujic Z
- Issue date: 2015 Sep 1
- Provocative decompression causes diffuse vascular injury in mice mediated by microparticles containing interleukin-1β.
- Authors: Thom SR, Bhopale VM, Yu K, Yang M
- Issue date: 2018 Aug 16
- Ascorbic acid supplementation diminishes microparticle elevations and neutrophil activation following SCUBA diving.
- Authors: Yang M, Barak OF, Dujic Z, Madden D, Bhopale VM, Bhullar J, Thom SR
- Issue date: 2015 Aug 15
- Separating the roles of nitrogen and oxygen in high pressure-induced blood-borne microparticle elevations, neutrophil activation, and vascular injury in mice.
- Authors: Yang M, Bhopale VM, Thom SR
- Issue date: 2015 Aug 1