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    Acute Elevated Glucose Promotes Abnormal Action Potential-Induced Ca2+ Transients in Cultured Skeletal Muscle Fibers

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    Author
    Hernández-Ochoa, E.O.
    Banks, Q.
    Schneider, M.F.
    Date
    2017
    Journal
    Journal of Diabetes Research
    Publisher
    Hindawi Limited
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1155/2017/1509048
    Abstract
    A common comorbidity of diabetes is skeletal muscle dysfunction, which leads to compromised physical function. Previous studies of diabetes in skeletal muscle have shown alterations in excitation-contraction coupling (ECC) - the sequential link between action potentials (AP), intracellular Ca2+ release, and the contractile machinery. Yet, little is known about the impact of acute elevated glucose on the temporal properties of AP-induced Ca2+ transients and ionic underlying mechanisms that lead to muscle dysfunction. Here, we used high-speed confocal Ca2+ imaging to investigate the temporal properties of AP-induced Ca2+ transients, an intermediate step of ECC, using an acute in cellulo model of uncontrolled hyperglycemia (25 mM, 48 h.). Control and elevated glucose-exposed muscle fibers cultured for five days displayed four distinct patterns of AP-induced Ca2+ transients (phasic, biphasic, phasic-delayed, and phasic-slow decay); most control muscle fibers show phasic AP-induced Ca2+ transients, while most fibers exposed to elevated D-glucose displayed biphasic Ca2+ transients upon single field stimulation. We hypothesize that these changes in the temporal profile of the AP-induced Ca2+ transients are due to changes in the intrinsic excitable properties of the muscle fibers. We propose that these changes accompany early stages of diabetic myopathy. Copyright 2017 Erick O. Hernández-Ochoa et al.
    Sponsors
    This work was supported by NIH Grant R37-AR055099 to Martin F. Schneider from the National Institute of Arthritis and Musculoskeletal and Skin Diseases.
    Keyword
    Action Potentials--drug effects
    Animals
    Calcium--metabolism
    Calcium Signaling--drug effects
    Excitation Contraction Coupling--drug effects
    Glucose--pharmacology
    Mice, Inbred C57BL
    Muscle Fibers, Skeletal--drug effects
    Muscle Fibers, Skeletal--metabolism
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85042743525&doi=10.1155%2f2017%2f1509048&partnerID=40&md5=53529a6ad2ad5a52972d8177c060f936; http://hdl.handle.net/10713/11004
    ae974a485f413a2113503eed53cd6c53
    10.1155/2017/1509048
    Scopus Count
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    UMB Open Access Articles 2017

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