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dc.contributor.authorEricksen, B.
dc.date.accessioned2019-09-19T18:35:43Z
dc.date.available2019-09-19T18:35:43Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85037707881&doi=10.12688%2ff1000research.5779.1&partnerID=40&md5=64354586c37d11186e4b79361d16511f
dc.identifier.urihttp://hdl.handle.net/10713/10915
dc.description.abstractDark blue rings and circles emerged when the non-specific polysaccharide stain lactophenol cotton blue was added to Gram stained slides. The dark blue staining is attributable to the presence of capsular polysaccharides and bacterial slime associated with clumps of Gram-negative bacteria. Since all bacterial cells are glycosylated and concentrate polysaccharides from the media, the majority of cells stain light blue. The contrast between dark and light staining is sufficient to enable a digital image processing thresholding technique to be quantitative with little background noise. Prior to the addition of lactophenol cotton blue, the Gram-stained slides appeared unremarkable, lacking ubiquitous clumps or stained polysaccharides. Adding lactophenol cotton blue to Gram stained slides is a quick and inexpensive way to screen cell cultures for bacterial slime, clumps and biofilms that are invisible using the Gram stain alone. The presence of cell clumping provides a possible explanation of the presence of persisters and paradoxical points observed in Virtual Colony Count antimicrobial assays, and suggests a phenotypic resistance mechanism to antimicrobial peptides involving capsular polysaccharides. Copyright 2017 Ericksen B.en_US
dc.description.urihttps://doi.org/10.12688/f1000research.5779.1en_US
dc.language.isoen_USen_US
dc.publisherFaculty of 1000 Ltden_US
dc.relation.ispartofF1000Research
dc.subjectantimicrobialsen_US
dc.subject.meshBiofilmsen_US
dc.subject.meshEscherichia colien_US
dc.subject.meshEnvironmenten_US
dc.titleQuantification of polysaccharides fixed to Gram stained slides using lactophenol cotton blue and digital image processingen_US
dc.typeArticleen_US
dc.identifier.doi10.12688/f1000research.5779.1


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