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    A multiplex qPCR approach for detection of pfhrp2 and pfhrp3 gene deletions in multiple strain infections of Plasmodium falciparum

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    Author
    Schindler, T.
    Deal A.C.
    Moser, K.A.
    Date
    2019
    Journal
    Scientific reports
    Publisher
    Nature Publishing Group
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1038/s41598-019-49389-2
    Abstract
    The rapid and accurate diagnosis of Plasmodium falciparum malaria infection is an essential factor in malaria control. Currently, malaria diagnosis in the field depends heavily on using rapid diagnostic tests (RDTs) many of which detect circulating parasite-derived histidine-rich protein 2 antigen (PfHRP2) in capillary blood. P. falciparum strains lacking PfHRP2, due to pfhrp2 gene deletions, are an emerging threat to malaria control programs. The novel assay described here, named qHRP2/3-del, is well suited for high-throughput screening of P. falciparum isolates to identify these gene deletions. The qHRP2/3-del assay identified pfhrp2 and pfhrp3 deletion status correctly in 93.4% of samples with parasitemia levels higher than 5 parasites/µL when compared to nested PCR. The qHRP2/3-del assay can correctly identify pfhrp2 and pfhrp3 gene deletions in multiple strain co-infections, particularly prevalent in Sub-Saharan countries. Deployment of this qHRP2/3-del assay will provide rapid insight into the prevalence and potential spread of P. falciparum isolates that escape surveillance by RDTs.
    Keyword
    qHRP2/3-del assay
    pfhrp2
    pfhrp3
    Malaria, Falciparum
    Gene Deletion
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072117221&doi=10.1038%2fs41598-019-49389-2&partnerID=40&md5=dc2331571b85a059843134f082fca902; http://hdl.handle.net/10713/10904
    ae974a485f413a2113503eed53cd6c53
    10.1038/s41598-019-49389-2
    Scopus Count
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    UMB Open Access Articles 2019

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