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    Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial

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    Author
    Machado, C.A.D.
    Souza, A.C.A.
    Martinho, F.C.
    Date
    2019
    Journal
    Brazilian oral research
    Publisher
    The Scientific Electronic Library Online
    Type
    Article
    
    Metadata
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    See at
    https://doi.org/10.1590/1807-3107bor-2019.vol33.0039
    Abstract
    This clinical study compared the effectiveness of two rotary systems: HyFlex CM (Coltene-Whaledent, Altstetten, Switzerland) and ProTaper Next (Dentsply Sirona, Ballaigues, Switzerland) on the removal of cultivable bacteria and endotoxins from primarily infected root canals. This study was designed as a randomized single-blinded, 2-arm, clinical trial. Twenty-four primarily infected root canals were selected and randomly divided into 2 groups: HyFlex CM (n = 12); and ProTaper Next (n = 12). Samples were collected before and after the biomechanical preparation and inoculated in specific flasks. Irrigation was performed using 2.5% sodium hypochlorite. A kinetic turbidimetric lysate assay of limulus amoebocytes was used to quantify endotoxins. Microbiological culture technique was used to determine the count of bacterial colony forming units (CFU/mL). Data collected were statistically analyzed using SigmaPlot 12.0 for Windows. The Two-Way ANOVA statistical test was performed and the level of significance was 5%. In the samples before the biomechanical preparation, cultivable bacteria and endotoxins were evidenced in 100% of the cases. The culture analysis revealed that there was no statistically significant difference in the bacterial reduction between the two instrumentation systems. Endotoxins were present in 100% of the canals after instrumentation and there was no statistical difference between the two systems in endotoxin reduction. Thus, it was concluded that both instrumentation systems were effective in reducing root canal bacteria and endotoxins with primary endodontic infection and that there was no statistical difference between them. However, no system was able to eliminate 100% of the bacteria and their by-products.
    Keyword
    endodontic rotary systems
    Root Canal Therapy
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85072011773&doi=10.1590%2f1807-3107bor-2019.vol33.0039&partnerID=40&md5=c7a9d97dfb61127e2d5ab4d01458aa9a; http://hdl.handle.net/10713/10861
    ae974a485f413a2113503eed53cd6c53
    10.1590/1807-3107bor-2019.vol33.0039
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    UMB Open Access Articles 2019

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